Marine Biotechnology

, Volume 6, Issue 5, pp 485–492

Fish as Bioreactors: Transgene Expression of Human Coagulation Factor VII in Fish Embryos

Authors

  • Gyulin Hwang
    • Division of Cell SciencesSchool of Biological Sciences, University of Southampton
  • Ferenc Müller
    • Division of Cell SciencesSchool of Biological Sciences, University of Southampton
    • Institute of Toxicology and GeneticsForschungszentrum Karlsruhe
  • M. Aziz. Rahman
    • Division of Cell SciencesSchool of Biological Sciences, University of Southampton
    • School of Chemical and Life SciencesUniversity of Greenwich at Medway
  • Darren W. Williams
    • Division of Cell SciencesSchool of Biological Sciences, University of Southampton
    • Department of BiologyUniversity of Washington
  • Paul J. Murdock
    • Haemophilia and Haemostasis CentreRoyal Free and UCL Medical School
  • K. John Pasi
    • Haemophilia and Haemostasis CentreRoyal Free and UCL Medical School
  • Geoffrey Goldspink
    • Department of SurgeryRoyal Free and UCL Medical School
  • Hamid Farahmand
    • Division of Cell SciencesSchool of Biological Sciences, University of Southampton
    • Division of Cell SciencesSchool of Biological Sciences, University of Southampton
Article

DOI: 10.1007/s10126-004-3121-2

Cite this article as:
Hwang, G., Müller, F., Rahman, M.A. et al. Mar Biotechnol (2004) 6: 485. doi:10.1007/s10126-004-3121-2

Abstract

A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.

Keywords

human coagulation factor VIItransgenic fishbioreactor.

Copyright information

© Springer-Verlag 2004