Marine Biotechnology

, Volume 7, Issue 3, pp 173–178

A Highly Active Alkaline Phosphatase from the Marine Bacterium Cobetia

Authors

  • E. Yu Plisova
    • Pacific Institute of Bioorganic ChemistryFar East Branch of the Russian Academy of Sciences
  • L.A. Balabanova
    • Pacific Institute of Bioorganic ChemistryFar East Branch of the Russian Academy of Sciences
  • E.P. Ivanova
    • Pacific Institute of Bioorganic ChemistryFar East Branch of the Russian Academy of Sciences
  • V. B. Kozhemyako
    • Pacific Institute of Bioorganic ChemistryFar East Branch of the Russian Academy of Sciences
  • V. V. Mikhailov
    • Pacific Institute of Bioorganic ChemistryFar East Branch of the Russian Academy of Sciences
  • E.V. Agafonova
    • Pacific Institute of Bioorganic ChemistryFar East Branch of the Russian Academy of Sciences
    • Pacific Institute of Bioorganic ChemistryFar East Branch of the Russian Academy of Sciences
Article

DOI: 10.1007/s10126-004-3022-4

Cite this article as:
Yu Plisova, E., Balabanova, L., Ivanova, E. et al. Mar Biotechnol (2005) 7: 173. doi:10.1007/s10126-004-3022-4

Abstract

An alkaline phosphatase with unusually high specific activity has been found to be produced by the marine bacterium Cobetia marina (strain KMM MC-296) isolated from coelomic liquid of the mussel Crenomytilus grayanus. The properties of enzyme, such as a very high specific activity (15000 DE U/1 mg of protein), no activation with divalent cations, resistance to high concentrations of inorganic phosphorus, as well as substrate specificity toward 5′ nucleotides suggest that the enzyme falls in an intermediate position between unspecific alkaline phosphatases (EC 3.1.3.1) and 5′ nucleotidases (EC 3.1.3.5).

Keywords

alkaline phosphatase purification properties specificity marine bacterium Cobetia marina.

Copyright information

© Springer Science+Business Media, Inc. 2005