Marine Biotechnology

, Volume 6, Issue 4, pp 335–346

Isolation and Characterization of a Fucoidan-Degrading Marine Bacterial Strain and Its Fucoidanase

Article

DOI: 10.1007/s10126-003-0033-5

Cite this article as:
Sakai, T., Kawai, T. & Kato, I. Mar. Biotechnol. (2004) 6: 335. doi:10.1007/s10126-003-0033-5

Abstract

A marine bacterial strain that degraded fucoidan from Kjellmaniella crassifolia (class Phaeophyceae, order Laminariales, family Laminariaceae) was isolated in our laboratory. The strain was gram-negative, ubiquinone 8 was the predominant respiratory quinone, and the GC-content of its genomic DNA was 36%. The cells of the strain were rod-shaped (2.0 μm long × 1.0 μm wide), and each cell was motile by means of one polar flagellum. Phylogenetic analysis of its 16S ribosomal DNA sequence indicated that it was a member of the family Alteromonadaceae. It produced a type of extracellular fucoidanase, an endosulfated fucan-digesting enzyme. The enzyme was purified with 3500-fold purity at 12.0% yield. Optimum conditions for the enzyme reaction were approximately pH 6.5 to 8.0 and temperature 30° to 35°C. The enzyme was activated by calcium ions, and maximum activity was observed in the presence of greater than 30 mM calcium ion.

Keywords

fucoidanasefucoidansulfated fucanAlteromonadaceaeKjellmaniella crassifoliasulfated fucose-containing polysaccharides.

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Takara Bio Inc., Biotechnology Research LaboratoriesOtsu-shiJapan