Marine Biotechnology

, Volume 5, Issue 1, pp 70–78

Marine Bacterial Sulfated Fucoglucuronomannan (SFGM) Lyase Digests Brown Algal SFGM into Trisaccharides

  • Takeshi Sakai
  • Hitomi Kimura
  • Kaoru Kojima
  • Kazuo Shimanaka
  • Katsushige Ikai
  • Ikunoshin Kato

DOI: 10.1007/s10126-002-0056-3

Cite this article as:
Sakai, T., Kimura, H., Kojima, K. et al. Mar. Biotechnol. (2003) 5: 70. doi:10.1007/s10126-002-0056-3

Abstract

Three kinds of trisaccharides were prepared by digesting fucoidan from the brown alga Kjellmaniella crassifolia, with the extracellular enzymes of the marine bacterium Fucobacter marina. Their structures were determined as Δ4,5GlcpUA1-2(L-Fucp(3-O-sulfate)α1-3)D-Manp, Δ4,5GlcpUA1-2(L-Fucp(3-O-sulfate)α1-3)D-Manp(6-O-sulfate), and Δ4,5GlcpUA1-2(L-Fucp(2,4-O-disulfate)α1-3)D-Manp(6-O-sulfate), which indicated the existence of a novel polysaccharide in the fucoidan and a novel glycosidase in the extracellular enzymes. In order to determine the complete structure of the polysaccharide and the reaction mechanism of the glycosidase, the fucoidan was partially hydrolyzed to obtain glucuronomannan, which is the putative backbone of the polysaccharide, and its sugar sequence was determined as (-4-D-GlcpUAβ1-2D-Manpα1-)n, which disclosed that the main structure of the polysaccharide is (-4-D-GlcpUAβ1-2(L-Fucp(3-O-sulfate)α1-3)D-Manpα1-)n. Consequently, the glycosidase was deduced to be an endo-α-D-mannosidase that eliminatively cleaves the α-D-mannosyl linkage between D-Manp and D-GlcpUA residues in the polysaccharide and produces the above trisaccharides. The novel polysaccharide and glycosidase were tentatively named as sulfated fucoglucuronomannan (SFGM) and SFGM lyase, respectively.

sulfated fucoglucuronomannan sulfated fucoglucuronomannan lyase Kjellmaniella crassifolia fucoidan sulfated fucose-containing polysaccharides

Copyright information

© Springer-Verlag New York Inc. 2003

Authors and Affiliations

  • Takeshi Sakai
    • 1
  • Hitomi Kimura
    • 1
  • Kaoru Kojima
    • 1
  • Kazuo Shimanaka
    • 2
  • Katsushige Ikai
    • 2
  • Ikunoshin Kato
    • 1
  1. 1.Takara Shuzo Co., Ltd., Biomedical Group, Zaifu-cho 82-4, Hirosaki-shi, Aomori-ken, 036-8216, Japan
  2. 2.Takara Shuzo Co., Ltd., Biomedical Group, Seta 3-4-1, Otsu-shi, Shiga-ken, 520-2193, Japan