Marine Biotechnology

, Volume 4, Issue 4, pp 347–355

Oligonucleotide Primers for PCR Amplification of Coelomate Introns

Authors

  • Simon N. Jarman
    • CSIRO Marine Research, GPO Box 1538, Hobart, TAS 7001, Australia
  • Robert D. Ward
    • CSIRO Marine Research, GPO Box 1538, Hobart, TAS 7001, Australia
  • Nicholas G. Elliott
    • CSIRO Marine Research, GPO Box 1538, Hobart, TAS 7001, Australia

DOI: 10.1007/s10126-002-0029-6

Cite this article as:
Jarman, S., Ward, R. & Elliott, N. Mar. Biotechnol. (2002) 4: 347. doi:10.1007/s10126-002-0029-6

Abstract Seven novel oligonucleotide primer pairs for polymerase chain reaction amplification of introns from nuclear genes in coelomates were designed and tested. Each pair bound to adjacent exons that are separated by a single intron in most coelomate species. The primer sets amplified introns in species as widely separated by the course of evolution as oysters (Mollusca: Protostoma) and salmon (Chordata: Deuterostoma). Each primer set was tested on a further 6 coelomate species and found to amplify introns in most cases. These primer sets may therefore be useful tools for developing nuclear DNA markers in diverse coelomate species for studies of population genetics, phylogenetics, or genome mapping.

Copyright information

© Springer-Verlag New York Inc. 2002