Marine Biotechnology

, Volume 4, Issue 4, pp 367–378

Genetic Diversity Within and Among Feral Populations and Domesticated Strains of the Guppy (Poecilia reticulata) in Singapore

  • Gideon  Khoo
  • Kok Fang  Lim
  • Damien K.Y. Gan
  • Fan  Chen
  • Woon-Khiong  Chan
  • Tit Meng  Lim
  • Violet P.E. Phang

DOI: 10.1007/s10126-002-0007-z

Cite this article as:
Khoo, G., Lim, K., Gan, D. et al. Mar. Biotechnol. (2002) 4: 367. doi:10.1007/s10126-002-0007-z

Genetic variability within and among feral populations and cultured strains of the guppy (Poecilia reticulata) was investigated by random amplification of polymorphic DNA (RAPD) fingerprinting. Feral guppies were collected from 6 isolated populations (BT, Bukit Timah; NS, Nee Soon; TS, Tuas; MF, Mount Faber; KR, Kranji; LI, laboratory-inbred feral line), while the Tuxedo and Green Variegated strains were sampled from 2 guppy farms in Singapore. Pairwise genetic distances analyzed by unweighted pair-group method with arithmetic means revealed distinct clustering of guppy individuals into their respective populations and strains. Percentage polymorphic loci ranged from 54.96% (TS) to 68.70% (KR), while average heterozygosity ranged from 0.220 (GV) to 0.271 (KR). In contrast, TS guppies had the highest (0.850) intrapopulation genetic similarity (S), whereas KR had the lowest (0.781). Among populations and strains, S ranged from 0.703 (between GV and LI) to 0.809 (between NS and MB). The GV strain S was closer to TX (0.784) than to the feral guppies. Bootstrapped genetic distance trees depicted 3 major nodes comprising BT-TS, NS-MF, and TX-GV. Principal coordinate analysis also differentiated the 6 feral populations from the 2 cultured strains.

Copyright information

© Springer-Verlag New York Inc. 2002

Authors and Affiliations

  • Gideon  Khoo
    • 1
  • Kok Fang  Lim
    • 1
  • Damien K.Y. Gan
    • 1
  • Fan  Chen
    • 1
  • Woon-Khiong  Chan
    • 1
  • Tit Meng  Lim
    • 1
  • Violet P.E. Phang
    • 1
  1. 1.Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Republic of SingaporeSG