Original Article

Lasers in Medical Science

, Volume 28, Issue 3, pp 901-909

Low-level laser therapy vs. pulsed electromagnetic field on neonatal rat calvarial osteoblast-like cells

  • Yusuf EmesAffiliated withDepartment of Oral and Maxillofacial Surgery, Faculty of Dentistry, Istanbul University
  • , Kivanç AkçaAffiliated withDepartment of Prosthodontics, Faculty of Dentistry, Hacettepe University
  • , Buket AybarAffiliated withDepartment of Oral and Maxillofacial Surgery, Faculty of Dentistry, Istanbul UniversityDepartment of Oral Surgery, Faculty of Dentistry, Istanbul University Email author 
  • , Serhat YalçınAffiliated withDepartment of Oral and Maxillofacial Surgery, Faculty of Dentistry, Istanbul University
  • , Yeliz ÇavuşoğluAffiliated withDepartment of Prosthodontics, Faculty of Dentistry, Hacettepe University
  • , Uğur BaysalAffiliated withDepartment of Electrical and Electronics Engineeering, Faculty of Engineering, Hacettepe University
  • , Halim IşseverAffiliated withDepartment of Public Health, Faculty of Medicine, Istanbul University
  • , Belir AtalayAffiliated withDepartment of Oral and Maxillofacial Surgery, Faculty of Dentistry, Istanbul University
  • , Pervin VuralAffiliated withDepartment of Biochemistry, Faculty of Medicine, Istanbul University
    • , Mine ErgüvenAffiliated withDepartment of Biochemistry, Faculty of Medicine, Yeni Yüzyıl University
    • , Murat Cavit ÇehreliAffiliated withDepartment of Prosthodontics, Faculty of Dentistry, Ordu University
    • , Ayhan BilirAffiliated withDepartment of Histology and Embriology, Faculty of Medicine, Istanbul University

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Abstract

To compare the effects of pulsed electromagnetic field (PEMF) and low-level laser therapy (LLLT) on osteoblast cells in a cell culture model. Fifty thousand neonatal rat calvarial osteoblast-like cells per milliliter were seeded and 0.06 mT PEMF, 0.2 mT PEMF, and LLLT at 808 nm were applied for 24 and 96 h on the cells. To evaluate cellular proliferation and differentiation, specimens were examined for DNA synthesis, alkaline phosphatase (ALP) activity, cell numbers, and viability of the cells. Morphological appearances of the cells were observed using scanning electron microcopy after 24 and 96 h of incubation. At 24 and 96 h, the control group had a higher cell proliferation than 0.06 and 0.2 mT PEMF groups (p = 0.001). At 96 h, 0.2 mT PEMF group had higher cell proliferation rate than 0.06 mT PEMF and LLLT groups (p = 0.001). The cell count and cell viability in 0.2 mT PEMF group were higher than the 0.06-mT PEMF and LLLT groups, although these differences were not statistically significant at 96 h (p > 0.05). At 24 and 96 h, cell viability in the control group was higher than the test groups. Alkaline phosphatase levels of the groups were comparable in both time intervals (p > 0.05). 0.2 mT PEMF application on osteoblast-like cells led to cell proliferation and differentiation better than 0.06 mT PEMF and LLLT at 808 nm, although a remarkable effect of both PEMF and LLLT could not be detected. The ALP activity of 0.2 and 0.06 mT PEMF and LLLT were comparable.

Keywords

Pulsed electromagnetic field Low-level laser therapy Osteoblast-like cells Cell proliferation and differentiation Alkaline phosphatase activity Scanning electron microscopy