Lasers in Medical Science

, Volume 28, Issue 3, pp 901–909

Low-level laser therapy vs. pulsed electromagnetic field on neonatal rat calvarial osteoblast-like cells

Authors

  • Yusuf Emes
    • Department of Oral and Maxillofacial Surgery, Faculty of DentistryIstanbul University
  • Kivanç Akça
    • Department of Prosthodontics, Faculty of DentistryHacettepe University
    • Department of Oral and Maxillofacial Surgery, Faculty of DentistryIstanbul University
    • Department of Oral Surgery, Faculty of DentistryIstanbul University
  • Serhat Yalçın
    • Department of Oral and Maxillofacial Surgery, Faculty of DentistryIstanbul University
  • Yeliz Çavuşoğlu
    • Department of Prosthodontics, Faculty of DentistryHacettepe University
  • Uğur Baysal
    • Department of Electrical and Electronics Engineeering, Faculty of EngineeringHacettepe University
  • Halim Işsever
    • Department of Public Health, Faculty of MedicineIstanbul University
  • Belir Atalay
    • Department of Oral and Maxillofacial Surgery, Faculty of DentistryIstanbul University
  • Pervin Vural
    • Department of Biochemistry, Faculty of MedicineIstanbul University
  • Mine Ergüven
    • Department of Biochemistry, Faculty of MedicineYeni Yüzyıl University
  • Murat Cavit Çehreli
    • Department of Prosthodontics, Faculty of DentistryOrdu University
  • Ayhan Bilir
    • Department of Histology and Embriology, Faculty of MedicineIstanbul University
Original Article

DOI: 10.1007/s10103-012-1165-5

Cite this article as:
Emes, Y., Akça, K., Aybar, B. et al. Lasers Med Sci (2013) 28: 901. doi:10.1007/s10103-012-1165-5

Abstract

To compare the effects of pulsed electromagnetic field (PEMF) and low-level laser therapy (LLLT) on osteoblast cells in a cell culture model. Fifty thousand neonatal rat calvarial osteoblast-like cells per milliliter were seeded and 0.06 mT PEMF, 0.2 mT PEMF, and LLLT at 808 nm were applied for 24 and 96 h on the cells. To evaluate cellular proliferation and differentiation, specimens were examined for DNA synthesis, alkaline phosphatase (ALP) activity, cell numbers, and viability of the cells. Morphological appearances of the cells were observed using scanning electron microcopy after 24 and 96 h of incubation. At 24 and 96 h, the control group had a higher cell proliferation than 0.06 and 0.2 mT PEMF groups (p = 0.001). At 96 h, 0.2 mT PEMF group had higher cell proliferation rate than 0.06 mT PEMF and LLLT groups (p = 0.001). The cell count and cell viability in 0.2 mT PEMF group were higher than the 0.06-mT PEMF and LLLT groups, although these differences were not statistically significant at 96 h (p > 0.05). At 24 and 96 h, cell viability in the control group was higher than the test groups. Alkaline phosphatase levels of the groups were comparable in both time intervals (p > 0.05). 0.2 mT PEMF application on osteoblast-like cells led to cell proliferation and differentiation better than 0.06 mT PEMF and LLLT at 808 nm, although a remarkable effect of both PEMF and LLLT could not be detected. The ALP activity of 0.2 and 0.06 mT PEMF and LLLT were comparable.

Keywords

Pulsed electromagnetic fieldLow-level laser therapyOsteoblast-like cellsCell proliferation and differentiationAlkaline phosphatase activityScanning electron microscopy

Copyright information

© Springer-Verlag London Ltd 2012