Lasers in Medical Science

, Volume 28, Issue 3, pp 901–909

Low-level laser therapy vs. pulsed electromagnetic field on neonatal rat calvarial osteoblast-like cells

  • Yusuf Emes
  • Kivanç Akça
  • Buket Aybar
  • Serhat Yalçın
  • Yeliz Çavuşoğlu
  • Uğur Baysal
  • Halim Işsever
  • Belir Atalay
  • Pervin Vural
  • Mine Ergüven
  • Murat Cavit Çehreli
  • Ayhan Bilir
Original Article

DOI: 10.1007/s10103-012-1165-5

Cite this article as:
Emes, Y., Akça, K., Aybar, B. et al. Lasers Med Sci (2013) 28: 901. doi:10.1007/s10103-012-1165-5

Abstract

To compare the effects of pulsed electromagnetic field (PEMF) and low-level laser therapy (LLLT) on osteoblast cells in a cell culture model. Fifty thousand neonatal rat calvarial osteoblast-like cells per milliliter were seeded and 0.06 mT PEMF, 0.2 mT PEMF, and LLLT at 808 nm were applied for 24 and 96 h on the cells. To evaluate cellular proliferation and differentiation, specimens were examined for DNA synthesis, alkaline phosphatase (ALP) activity, cell numbers, and viability of the cells. Morphological appearances of the cells were observed using scanning electron microcopy after 24 and 96 h of incubation. At 24 and 96 h, the control group had a higher cell proliferation than 0.06 and 0.2 mT PEMF groups (p = 0.001). At 96 h, 0.2 mT PEMF group had higher cell proliferation rate than 0.06 mT PEMF and LLLT groups (p = 0.001). The cell count and cell viability in 0.2 mT PEMF group were higher than the 0.06-mT PEMF and LLLT groups, although these differences were not statistically significant at 96 h (p > 0.05). At 24 and 96 h, cell viability in the control group was higher than the test groups. Alkaline phosphatase levels of the groups were comparable in both time intervals (p > 0.05). 0.2 mT PEMF application on osteoblast-like cells led to cell proliferation and differentiation better than 0.06 mT PEMF and LLLT at 808 nm, although a remarkable effect of both PEMF and LLLT could not be detected. The ALP activity of 0.2 and 0.06 mT PEMF and LLLT were comparable.

Keywords

Pulsed electromagnetic fieldLow-level laser therapyOsteoblast-like cellsCell proliferation and differentiationAlkaline phosphatase activityScanning electron microscopy

Copyright information

© Springer-Verlag London Ltd 2012

Authors and Affiliations

  • Yusuf Emes
    • 1
  • Kivanç Akça
    • 2
  • Buket Aybar
    • 1
    • 9
  • Serhat Yalçın
    • 1
  • Yeliz Çavuşoğlu
    • 2
  • Uğur Baysal
    • 3
  • Halim Işsever
    • 4
  • Belir Atalay
    • 1
  • Pervin Vural
    • 5
  • Mine Ergüven
    • 6
  • Murat Cavit Çehreli
    • 7
  • Ayhan Bilir
    • 8
  1. 1.Department of Oral and Maxillofacial Surgery, Faculty of DentistryIstanbul UniversityIstanbulTurkey
  2. 2.Department of Prosthodontics, Faculty of DentistryHacettepe UniversityAnkaraTurkey
  3. 3.Department of Electrical and Electronics Engineeering, Faculty of EngineeringHacettepe UniversityAnkaraTurkey
  4. 4.Department of Public Health, Faculty of MedicineIstanbul UniversityIstanbulTurkey
  5. 5.Department of Biochemistry, Faculty of MedicineIstanbul UniversityIstanbulTurkey
  6. 6.Department of Biochemistry, Faculty of MedicineYeni Yüzyıl UniversityIstanbulTurkey
  7. 7.Department of Prosthodontics, Faculty of DentistryOrdu UniversityOrduTurkey
  8. 8.Department of Histology and Embriology, Faculty of MedicineIstanbul UniversityIstanbulTurkey
  9. 9.Department of Oral Surgery, Faculty of DentistryIstanbul UniversityÇapa/İstanbulTurkey