Lasers in Medical Science

, Volume 27, Issue 1, pp 237–249

Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells

  • Khalid M. AlGhamdi
  • Ashok Kumar
  • Noura A. Moussa
Review Article

DOI: 10.1007/s10103-011-0885-2

Cite this article as:
AlGhamdi, K.M., Kumar, A. & Moussa, N.A. Lasers Med Sci (2012) 27: 237. doi:10.1007/s10103-011-0885-2

Abstract

The aim of this work is to review the available literature on the details of low-level laser therapy (LLLT) use for the enhancement of the proliferation of various cultured cell lines including stem cells. A cell culture is one of the most useful techniques in science, particularly in the production of viral vaccines and hybrid cell lines. However, the growth rate of some of the much-needed mammalian cells is slow. LLLT can enhance the proliferation rate of various cell lines. Literature review from 1923 to 2010. By investigating the outcome of LLLT on cell cultures, many articles report that it produces higher rates of ATP, RNA, and DNA synthesis in stem cells and other cell lines. Thus, LLLT improves the proliferation of the cells without causing any cytotoxic effects. Mainly, helium neon and gallium-aluminum-arsenide (Ga-Al-As) lasers are used for LLLT on cultured cells. The results of LLLT also vary according to the applied energy density and wavelengths to which the target cells are subjected. This review suggests that an energy density value of 0.5 to 4.0 J/cm2 and a visible spectrum ranging from 600 to 700 nm of LLLT are very helpful in enhancing the proliferation rate of various cell lines. With the appropriate use of LLLT, the proliferation rate of cultured cells, including stem cells, can be increased, which would be very useful in tissue engineering and regenerative medicine.

Keywords

Low-level laser therapy Cell culture Stem cells Proliferation Tissue engineering Regenerative medicine 

Copyright information

© Springer-Verlag London Ltd 2011

Authors and Affiliations

  • Khalid M. AlGhamdi
    • 1
  • Ashok Kumar
    • 2
  • Noura A. Moussa
    • 3
  1. 1.Department of Dermatology, Vitiligo Research Chair, College of MedicineKing Saud UniversityRiyadhSaudi Arabia
  2. 2.Vitiligo Research Chair, College of MedicineKing Saud UniversityRiyadhSaudi Arabia
  3. 3.Vitiligo Research Chair, College of MedicineKing Saud UniversityRiyadhSaudi Arabia

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