Lasers in Medical Science

, Volume 25, Issue 5, pp 705–710

Antimicrobial effect of photodynamic therapy using a highly pure chlorin e6

Authors

  • Jong-Hwan Park
    • Department of Biochemistry, College of MedicineKonyang University
  • Yeon-Hee Moon
    • Department of Pathology, School of DentistryChosun University
  • Iel-Soo Bang
    • Microbiology & Immunology, School of DentistryChosun University
  • Yong-Chul Kim
    • Department of Life ScienceGIST
  • Soo-A Kim
    • Department of Biochemistry, College of Oriental MedicineDongguk University
    • Department of Pathology, School of DentistryChosun University
    • Department of Pathology, School of DentistryChosun University
Original Article

DOI: 10.1007/s10103-010-0781-1

Cite this article as:
Park, J., Moon, Y., Bang, I. et al. Lasers Med Sci (2010) 25: 705. doi:10.1007/s10103-010-0781-1

Abstract

The aim of the present study is to evaluate the antimicrobial effect of photodynamic therapy (PDT) using a highly pure chlorin e6 (Ce6), against various pathogenic bacteria. To examine the antimicrobial effect of Ce6-mediated PDT against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Salmonella enterica serovar Typhimurium, inhibition zone formation, CFU quantification, and bacterial viability were evaluated. Inhibition zone analysis showed that Ce6-mediated PDT is very effective to inhibit the growth of S. aureus and P. aeruginosa, but has only minor effect to E. coli and S. Typhimurium, which was dependent on the energy density of laser and dose of Ce6. Ce6-mediated PDT also nearly inhibited the colony formation of S. aureus and P. aeruginosa, and partially inhibited that of E. coli and S. Typhimurium. In addition, the number of viable bacteria decreased greatly after PDT application with LS-chlorin e6 of 10 μM and laser and energy density of 20 J/cm2. These results show that Ce6-mediated PDT can be an effective alternative for antimicrobial treatment.

Keywords

Photodynamic therapyChlorin e6Inhibition zone formationBacterial viabilityAntimicrobial treatment

Copyright information

© Springer-Verlag London Ltd 2010