Lasers in Medical Science

, Volume 24, Issue 2, pp 209–213

Low-level laser therapy (670 nm) on viability of random skin flap in rats

  • Paulo Sérgio Bossini
  • Renan Fangel
  • Rafael Malfará Habenschus
  • Ana Claudia Renno
  • Benedito Benze
  • José Antônio Zuanon
  • Carlos Benatti Neto
  • Nivaldo Antonio Parizotto
Original Article

DOI: 10.1007/s10103-008-0551-5

Cite this article as:
Bossini, P.S., Fangel, R., Habenschus, R.M. et al. Lasers Med Sci (2009) 24: 209. doi:10.1007/s10103-008-0551-5

Abstract

This study investigated the effects of 670 nm laser, at different fluences, on the viability of skin flap in rats. One hundred male animals were used. The animals were divided into control group; group treated with 3 J/cm2; group treated with 6 J/cm2; group treated with 12 J/cm2 and group treated with 24 J/cm2. The skin flap was made on the backs of all animals studied, with a plastic sheet interposed between the flap and the donor site. Laser irradiation was done immediately after the surgery and on days 1, 2, 3 and 4 after surgery. The percentage of necrosis of the flap was calculated at the 7th postoperative day. Additionally, a sample of each flap was collected to enable us to count the blood vessels. Treated animals showed a statistically significant smaller area of necrosis than did the control group. The necrosis in the treated groups was 41.82% (group 2), 36.51% (group 3), 29.45% (group 4) and 20.37% (group 5). We also demonstrated that laser irradiation at 670 nm, at all doses used, had a stimulatory effect on angiogenesis. Our study showed that the 670 nm laser was efficient to increase the viability of the skin flap, at all fluences used, with a tendency of reaching better results at higher doses.

Keywords

Laser therapyMicrocirculationNecrosisSurgical flaps

Copyright information

© Springer-Verlag London Ltd 2008

Authors and Affiliations

  • Paulo Sérgio Bossini
    • 1
  • Renan Fangel
    • 1
  • Rafael Malfará Habenschus
    • 1
  • Ana Claudia Renno
    • 2
    • 5
  • Benedito Benze
    • 3
  • José Antônio Zuanon
    • 4
  • Carlos Benatti Neto
    • 4
  • Nivaldo Antonio Parizotto
    • 1
  1. 1.Department of PhysiotherapyFederal University of São CarlosSao CarlosBrazil
  2. 2.Department of Health ScienceFederal University of Sao PauloSantosBrazil
  3. 3.Department of StatisticFederal University of São CarlosSao CarlosBrazil
  4. 4.Department of Physiology and PathologyEstadual University of the State of Sao Paulo (UNESP)AraraquaraBrazil
  5. 5.Department of PhysiotherapyFederal University of São PauloSantosBrazil