Clean Technologies and Environmental Policy

, Volume 16, Issue 1, pp 11–21

Biohydrogen production from agroindustrial wastes via Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)

  • Najeeb Kaid Nasser Al-Shorgani
  • El-Mubarak Tibin
  • Ehsan Ali
  • Aidil Abdul Hamid
  • Wan Mohtar Wan Yusoff
  • Mohd Sahaid Kalil
Original Paper

DOI: 10.1007/s10098-013-0586-6

Cite this article as:
Al-Shorgani, N.K.N., Tibin, EM., Ali, E. et al. Clean Techn Environ Policy (2014) 16: 11. doi:10.1007/s10098-013-0586-6

Abstract

The anaerobic production of biohydrogen from different pretreated agroindustrial wastes, including rice bran (RB), de-oiled RB (DRB), sago starch (SS), and palm oil mill effluent (POME) via Clostridium saccharoperbutylacetonicum N1-4 was investigated in a batch culture system at 30 °C and a pH of 6.2. A yield of 7627, 6995, and 6,363 mL H2/L was obtained from H2SO4 (1 %)-treated DRB (10 %), enzymatically hydrolyzed DRB (10 %) and HCl (1 %)-treated DRB (10 %), respectively; however, untreated DRB (10 %) was able to produce only 3,286 mL H2/L. A strategic treatment of RB (10 %) with HCl (1 %) followed by enzymatic hydrolysis could produce 3,172 mL H2/L. An enzymatically hydrolyzed mixture of each POME and SS (5 %) produced 3,474 mL H2/L, and a remarkable enhancement of H2 production (7,020 mL H2/L) was achieved when the same mixture was subjected to XAD-4 resin treatment. In contrast, the enzymatically hydrolyzed SS (5 %) could produce only 4,628 mL H2/L. Conclusively, it can be stated that agricultural wastes have a potential as substrates for biohydrogen production and that pretreatment with C. saccharoperbutylacetonicum N1-4 can contribute positively to enhancing the production.

Keywords

Biohydrogen Clostridium saccharoperbutylacetonicum N1-4 Anaerobic fermentation Agroindustrial wastes Renewable energy 

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Najeeb Kaid Nasser Al-Shorgani
    • 1
    • 4
  • El-Mubarak Tibin
    • 1
  • Ehsan Ali
    • 2
  • Aidil Abdul Hamid
    • 1
  • Wan Mohtar Wan Yusoff
    • 1
  • Mohd Sahaid Kalil
    • 3
  1. 1.School of Biosciences and Biotechnology, Faculty of Sciences and TechnologyUniversiti Kebangsaan MalaysiaBangiMalaysia
  2. 2.Centre for Energy SystemsNational University of Sciences and TechnologyIslamabadPakistan
  3. 3.Department of Chemical and Process Engineering, Faculty of EngineeringUniversiti Kebangsaan MalaysiaBangiMalaysia
  4. 4.Department of Applied Microbiology, Faculty of Applied SciencesTaiz UniversityTaizYemen

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