European Journal of Clinical Microbiology and Infectious Diseases

, Volume 20, Issue 9, pp 666–669

Determination of HCV Genotype Using Two Antibody Assays and Genome Typing

Authors

  •  K. Sandres
    • Service de Virologie, Hôpital PURPAN, Place du Dr Baylac, 31059 Toulouse Cedex, France
  •  M. Dubois
    • Service de Virologie, Hôpital PURPAN, Place du Dr Baylac, 31059 Toulouse Cedex, France
  •  C. Pasquier
    • Service de Virologie, Hôpital PURPAN, Place du Dr Baylac, 31059 Toulouse Cedex, France
  •  J. Puel
    • Service de Virologie, Hôpital PURPAN, Place du Dr Baylac, 31059 Toulouse Cedex, France
  •  J. Izopet
    • Service de Virologie, Hôpital PURPAN, Place du Dr Baylac, 31059 Toulouse Cedex, France
Concise Article

DOI: 10.1007/s100960100576

Cite this article as:
Sandres, K., Dubois, M., Pasquier, C. et al. Eur J Clin Microbiol Infect Dis (2001) 20: 666. doi:10.1007/s100960100576

Abstract.

Two serotyping assays for hepatitis C virus (Serotyping 1–6 assay; Murex, UK and RIBA Serotyping SI; Chiron, USA) were compared to a standardized genotyping assay (Inno-LiPA HCV II; Innogenetics, Belgium) using serum samples collected from 126 patients chronically infected with hepatitis C virus. Serotyping was positive in 87% and 80% of the samples tested with Murex and Chiron, respectively, and concordant with genotyping in 93% and 88%, respectively. Sequence analysis of the NS5b region of 15 samples with discrepant typing results confirmed the Inno-LiPA finding in all instances. The Murex enzyme immunoassay serotyping method was less sensitive for identifying genotype 2 (P<0.05). The concordance with genotyping of the RIBA serotyping method was lower for genotype 2 than for the other genotypes (P<0.05).

Download to read the full article text

Copyright information

© Springer-Verlag 2001