Determination of HCV Genotype Using Two Antibody Assays and Genome Typing
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- Sandres, K., Dubois, M., Pasquier, C. et al. Eur J Clin Microbiol Infect Dis (2001) 20: 666. doi:10.1007/s100960100576
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Two serotyping assays for hepatitis C virus (Serotyping 1–6 assay; Murex, UK and RIBA Serotyping SI; Chiron, USA) were compared to a standardized genotyping assay (Inno-LiPA HCV II; Innogenetics, Belgium) using serum samples collected from 126 patients chronically infected with hepatitis C virus. Serotyping was positive in 87% and 80% of the samples tested with Murex and Chiron, respectively, and concordant with genotyping in 93% and 88%, respectively. Sequence analysis of the NS5b region of 15 samples with discrepant typing results confirmed the Inno-LiPA finding in all instances. The Murex enzyme immunoassay serotyping method was less sensitive for identifying genotype 2 (P<0.05). The concordance with genotyping of the RIBA serotyping method was lower for genotype 2 than for the other genotypes (P<0.05).