In Vitro Adherence of Enterococcus faecalis and Enterococcus faecium to Urinary Catheters

  • P. Joyanes
  • A. Pascual
  • L. Martínez-Martínez
  • A. Hevia
  • E. J. Perea
Note

DOI: 10.1007/s100960050443

Cite this article as:
Joyanes, P., Pascual, A., Martínez-Martínez, L. et al. EJCMID (2000) 19: 124. doi:10.1007/s100960050443

Abstract

 The in vitro adherence of ten strains of Enterococcus faecalis and ten strains of Enterococcus faecium to siliconized latex urinary catheters and to silicone elastomer was evaluated. Bacterial suspensions (2.5×105 cfu/ml) in tryptic soy broth containing 0.5 cm segments from each type of catheter were incubated at 37 °C. At specified intervals, the segments were washed to remove nonadherent bacteria and sonicated for 1 min, and colony-forming units were quantified. Bacterial adherence occurred rapidly, reaching maximal peaks after 24 h of incubation. Enterococcus faecium adherence to both biomaterials was significantly lower than that of Enterococcus faecalis. No differences were observed between the two elastomers. Bacterial adherence was not related to bacterial surface hydrophobicity, hemolysin or gelatinase production.

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • P. Joyanes
    • 2
  • A. Pascual
    • 2
  • L. Martínez-Martínez
    • 2
  • A. Hevia
    • 1
  • E. J. Perea
    • 2
  1. 1.Department of Pathology, School of Medicine, Seville, SpainES
  2. 2.Department of Microbiology, School of Medicine, Apartado 914, 41080 Seville, Spain e-mail: atomas@cica.esES