European Journal of Clinical Microbiology and Infectious Diseases

, Volume 19, Issue 8, pp 627–630

Molecular Typing of Bacteria Directly from Cerebrospinal Fluid

Authors

  • M. C. Enright
    • Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, South Parks Road, University of Oxford, Oxford OX1 3FY, UK e-mail: mark.enright@ceid.ox.ac.uk
  • K. Knox
    • Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, South Parks Road, University of Oxford, Oxford OX1 3FY, UK e-mail: mark.enright@ceid.ox.ac.uk
  • D. Griffiths
    • Department of Microbiology, John Radcliffe Hospital, Oxford University, Oxford OX3 9DU, UK
  • D. W. M. Crook
    • Department of Microbiology, John Radcliffe Hospital, Oxford University, Oxford OX3 9DU, UK
  • B. G. Spratt
    • Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, South Parks Road, University of Oxford, Oxford OX1 3FY, UK e-mail: mark.enright@ceid.ox.ac.uk
Note

DOI: 10.1007/s100960000321

Cite this article as:
Enright, M., Knox, K., Griffiths, D. et al. EJCMID (2000) 19: 627. doi:10.1007/s100960000321

Abstract

 Using Streptococcus pneumoniae as an example, the ability of multilocus sequence typing (MLST) to characterise isolates directly from cerebrospinal fluid (CSF) was investigated. A nested multiplex polymerase chain reaction method that amplifies the seven housekeeping gene fragments used for pneumococcal MLST was applied to 30 CSF samples from suspected cases of bacterial meningitis. The fragments were amplified from all 14 samples from which Streptococcus pneumoniae was cultured, and, after direct sequencing, the allelic profiles obtained from ten of the samples corresponded to those of clones previously associated with invasive pneumococcal disease. MLST could also predict the penicillin susceptibility and serotype of the CSF isolates.

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© Springer-Verlag Berlin Heidelberg 2000