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An abbreviated MLVA identifies Escherichia coli ST131 as the major extended-spectrum β-lactamase-producing lineage in the Copenhagen area

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Abstract

Rapid bacterial typing is a valuable and necessary tool in the prevention and detection of outbreaks. The purpose of this study was to adapt a multilocus variable number of tandem repeats analysis (MLVA) for analysis on a benchtop capillary electrophoresis instrument and compare the modified assay with multilocus sequence typing (MLST) for typing cefpodoxime-resistant Escherichia coli (E. coli). Further, we identified the causative resistance mechanisms and epidemiological type of infection for isolates producing extended-spectrum β-lactamases (ESBLs). A collection of E. coli resistant to cefpodoxime was typed by MLST and a modified MLVA assay using a benchtop capillary electrophoresis instrument. Resistance mechanisms were identified by polymerase chain reaction (PCR) and sequencing. Patient history was examined to establish the epidemiological type of infection for ESBL-producing E. coli. MLVA yielded typing results homologous with MLST and it correctly identified E. coli sequence type (ST) 131 that was accounting for 45 % of all ESBL-producing isolates in the sample collection. The majority (76.7 %) of ESBL-producing isolates was healthcare-related and only 23.3 % of the ESBL-producing isolates were community-onset infections (COI), regardless of the ST. Patients with COI were significantly more often of female gender and younger age compared to healthcare-associated infections (HCAI) and hospital-onset infections (HOI). In conclusion, the modified MLVA is a useful tool for the rapid typing of E. coli and it identified ST131 as the predominating ESBL-producing lineage in Copenhagen. Healthcare-related infections were the predominant infection setting of ESBL-producing E. coli and the demographic characteristics differed between patients with COI and healthcare-related infections.

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Acknowledgment

This work was supported by Fondation Idella (3.3.5/2008/II to K.S.), Fonden til Lægevidenskabens Fremme (10–316 to K.S.), and Hvidovre Hospitals Forskningsfond (grant to J.B.N.).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Clinical Microbiology 445, Hvidovre Hospital, Kettegaard Alle 30, 2650, Hvidovre, Denmark

    J. B. Nielsen, A. Albayati, R. L. Jørgensen, K. H. Hansen, B. Lundgren & K. Schønning

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  1. J. B. Nielsen
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  2. A. Albayati
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  3. R. L. Jørgensen
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  4. K. H. Hansen
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Correspondence to J. B. Nielsen.

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Nielsen, J.B., Albayati, A., Jørgensen, R.L. et al. An abbreviated MLVA identifies Escherichia coli ST131 as the major extended-spectrum β-lactamase-producing lineage in the Copenhagen area. Eur J Clin Microbiol Infect Dis 32, 431–436 (2013). https://doi.org/10.1007/s10096-012-1764-x

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  • DOI: https://doi.org/10.1007/s10096-012-1764-x

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