European Journal of Clinical Microbiology & Infectious Diseases

, Volume 27, Issue 12, pp 1209–1217

Liquid culture medium for the rapid cultivation of Helicobacter pylori from biopsy specimens

  • N. Sainsus
  • V. Cattori
  • C. Lepadatu
  • R. Hofmann-Lehmann
Article

DOI: 10.1007/s10096-008-0567-6

Cite this article as:
Sainsus, N., Cattori, V., Lepadatu, C. et al. Eur J Clin Microbiol Infect Dis (2008) 27: 1209. doi:10.1007/s10096-008-0567-6

Abstract

The goal of this study was to develop a liquid culture medium for the rapid isolation, cultivation, identification and subsequent antibiotics susceptibility testing of Helicobacter pylori directly from biopsy specimens. Five liquid media were tested: Ham’s F-12, Brucella broth, tryptic soybroth, brain heart infusion broth and Mueller-Hinton broth. After optimisation of the medium, it was applied in order to investigate biopsy samples from 150 patients with gastro-duodenal disorders and compared with traditional culture methods, microscopy and an H. pylori-specific TaqMan real-time polymerase chain reaction (PCR). The most reliable and rapid growth of H. pylori, even at a small inoculum size, was obtained in Ham’s F-12 medium with 5% horse serum. The developed system allowed the primary isolation of H. pylori in clinical samples and provided 87% sensitivity and 100% specificity.

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • N. Sainsus
    • 1
  • V. Cattori
    • 2
  • C. Lepadatu
    • 3
  • R. Hofmann-Lehmann
    • 2
  1. 1.Department of MicrobiologyState University of Medicine and Pharmacy “N. Testemiţanu”ChishinauMoldova
  2. 2.Clinical Laboratory, Vetsuisse FacultyUniversity of ZurichZurichSwitzerland
  3. 3.Department of Surgery, Faculty of CME, Clinical Republican HospitalState University of Medicine and Pharmacy “N. Testemiţanu”ChishinauMoldova