European Journal of Clinical Microbiology & Infectious Diseases

, Volume 26, Issue 3, pp 221–224

Comparison of PCR, microscopic examination and culture for the early diagnosis and characterization of Acanthamoeba isolates from ocular infections

  • H. Yera
  • O. Zamfir
  • T. Bourcier
  • T. Ancelle
  • L. Batellier
  • J. Dupouy-Camet
  • C. Chaumeil
Concise Article

DOI: 10.1007/s10096-007-0268-6

Cite this article as:
Yera, H., Zamfir, O., Bourcier, T. et al. Eur J Clin Microbiol Infect Dis (2007) 26: 221. doi:10.1007/s10096-007-0268-6

Abstract

In the study presented here, PCR, microscopic examination and culture of corneal samples were compared as methods of confirming the clinical diagnosis of Acanthamoeba keratitis, a serious ocular infection that is difficult to diagnose and threatens eyesight. The three methods were applied to isolates obtained from 513 patients with clinical signs or risk factors suggesting Acanthamoeba infection. Acanthamoeba keratitis was diagnosed in 12 of these patients. Combined PCR assays were more sensitive (94%) than either microscopic examination (33%) or culture (7%). The Acanthamoeba isolates were characterized using DNA sequence analysis of the nuclear small-subunit rRNA gene, and T4 was the predominant genotype found.

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • H. Yera
    • 1
  • O. Zamfir
    • 2
  • T. Bourcier
    • 3
  • T. Ancelle
    • 1
  • L. Batellier
    • 2
  • J. Dupouy-Camet
    • 1
  • C. Chaumeil
    • 2
  1. 1.Laboratoire de Parasitologie Mycologie, Hôpital Cochin, AP-HPUniversité Paris DescartesParis Cedex 14France
  2. 2.Laboratoire, Centre Hospitalier National d’Ophtalmologie des Quinze-VingtsParis Cedex 12France
  3. 3.Service du Professeur Laurent Laroche, Centre Hospitalier National d’Ophtalmologie des Quinze-VingtsParis Cedex 12France