Rapid detection of Panton–Valentine leukocidin-positive Staphylococcus aureus by real-time PCR targeting the lukS-PV gene
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- Reischl, U., Tuohy, M.J., Hall, G.S. et al. Eur J Clin Microbiol Infect Dis (2007) 26: 131. doi:10.1007/s10096-007-0254-z
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In order to assess the speed and accuracy of a real-time PCR assay targeting the lukS-PV gene of Panton–Valentine leukocidin (PVL)-positive Staphylococcus aureus, 700 S. aureus strains were tested and the results were compared to those achieved with block cycler PCR. Cross-reactivity was tested with 166 other bacterial species. Using this homogeneous real-time PCR assay format, the presence or absence of genetic information for PVL, which is also found in community-associated methicillin-resistant S. aureus, was correctly identified from pure culture and directly in various types of clinical specimens.