Rapid detection of Panton–Valentine leukocidin-positive Staphylococcus aureus by real-time PCR targeting the lukS-PV gene

  • U. Reischl
  • M. J. Tuohy
  • G. S. Hall
  • G. W. Procop
  • N. Lehn
  • H. Linde
Concise Article

DOI: 10.1007/s10096-007-0254-z

Cite this article as:
Reischl, U., Tuohy, M.J., Hall, G.S. et al. Eur J Clin Microbiol Infect Dis (2007) 26: 131. doi:10.1007/s10096-007-0254-z

Abstract

In order to assess the speed and accuracy of a real-time PCR assay targeting the lukS-PV gene of Panton–Valentine leukocidin (PVL)-positive Staphylococcus aureus, 700 S. aureus strains were tested and the results were compared to those achieved with block cycler PCR. Cross-reactivity was tested with 166 other bacterial species. Using this homogeneous real-time PCR assay format, the presence or absence of genetic information for PVL, which is also found in community-associated methicillin-resistant S. aureus, was correctly identified from pure culture and directly in various types of clinical specimens.

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • U. Reischl
    • 1
  • M. J. Tuohy
    • 2
  • G. S. Hall
    • 2
  • G. W. Procop
    • 2
  • N. Lehn
    • 1
  • H. Linde
    • 1
  1. 1.Institute of Medical Microbiology and HygieneUniversity Hospital of RegensburgRegensburgGermany
  2. 2.Clinical MicrobiologyCleveland Clinic FoundationClevelandUSA