Neurological Sciences

, Volume 31, Issue 3, pp 283–291

CSF proteomic analysis in patients with normal pressure hydrocephalus selected for the shunt: CSF biomarkers of response to surgical treatment

Authors

    • Department of NeurosurgeryUniversity of Florence
  • Alessandro Terreni
    • Department of Laboratory DiagnosticCareggi University Hospital of Florence
  • Anna Caldini
    • Department of Laboratory DiagnosticCareggi University Hospital of Florence
  • Benedetta Salvadori
    • Department of Laboratory DiagnosticCareggi University Hospital of Florence
  • Pasquale Gallina
    • Department of NeurosurgeryUniversity of Florence
  • Simona Francese
    • Mass Spectrometry Center of the University of Florence
  • Guido Mastrobuoni
    • Mass Spectrometry Center of the University of Florence
  • Giuseppe Pieraccini
    • Mass Spectrometry Center of the University of Florence
  • Gloriano Moneti
    • Mass Spectrometry Center of the University of Florence
  • Luca Bini
    • Functional Proteomics Laboratory, Department of Molecular BiologyUniversity of Siena
  • Gianni Messeri
    • Department of Laboratory DiagnosticCareggi University Hospital of Florence
  • Nicola Di Lorenzo
    • Department of NeurosurgeryUniversity of Florence
Original Article

DOI: 10.1007/s10072-009-0181-0

Cite this article as:
Scollato, A., Terreni, A., Caldini, A. et al. Neurol Sci (2010) 31: 283. doi:10.1007/s10072-009-0181-0

Abstract

The aim of our pilot study was to investigate, by a proteomic approach, the expressed differences in cerebrospinal fluid (CSF) protein patterns in order to aid in the diagnosis and treatment of normal pressure hydrocephalus (NPH). Seventeen patients with NPH, selected by Intracranial-Pressure monitoring (ICPmo), underwent implantation of a shunt and after 6 months were clinically re-evaluated. Thirteen patients improved, whereas four did not. During ICPmo CSF was collected and its proteoma was analyzed by 2D gel electrophoresis and mass spectrometry. The over-expression of α2HS glycoprotein, α1 antichimotrypsin and α1beta glycoprotein and the under-expression of glial fibrillary acidic protein, apolipoproteins (AIV, J and E), complement C3c, anti-thrombin, α2 antiplasmin and albumin seem to be associated with a positive response to surgery. Most of these proteins have been reported to be altered in Alzheimer disease, supporting the hypothesis of a possible link between these two nosological entities.

Keywords

Normal pressure hydrocephalusCerebrospinal fluidProteoma2D-electrophoresis

Copyright information

© Springer-Verlag 2009