Food Science and Biotechnology

, Volume 19, Issue 2, pp 367–371

Isolation and preliminary identification of a novel microorganism producing aspartame from soil samples

  • Jian Dong Cui
  • Ya Nan Zhang
  • Gui Xia Zhao
  • Guo Qun Zhao
  • Si Zhang
  • Jun Lu
  • Shi Ru Jia
Research Article

DOI: 10.1007/s10068-010-0052-8

Cite this article as:
Cui, J.D., Zhang, Y.N., Zhao, G.X. et al. Food Sci Biotechnol (2010) 19: 367. doi:10.1007/s10068-010-0052-8

Abstract

To develop a new method for producing aspartame (APM), a simple and efficient strategy for the isolation of certain microorganisms producing APM from soil samples was designed. A newly strain with secreting certain specific dipeptidase to directly synthesize APM from the substrates of l-aspartic acid (l-Asp) and l-phenylalanine methyl ester (l-PM) without protection for amino acid side chains was screened from soil samples. APM concentration in reaction mixture was quantified by high performance liquid chromatography (HPLC), yield reached 0.015 g/L. Examination of the general morphological characteristics and data from the sequence analysis of the rDNA-ITS gene led to identification of the isolate as a strain of fungal endophyte spp. The newly isolated strain had a high potential for application in industrial processes for APM production. In particular, this new method was low cost for synthesis of APM during the reaction due to avoiding protection for amino acid side chains and optical resolution of the mixtures. As we known it, this is first report that a newly strain with a high potential for selective synthesis of the APM was isolated from soil.

Keywords

aspartame biosynthesis isolation identification fungal endophyte spp 

Copyright information

© The Korean Society of Food Science and Technology and Springer Netherlands 2010

Authors and Affiliations

  • Jian Dong Cui
    • 1
    • 4
  • Ya Nan Zhang
    • 2
  • Gui Xia Zhao
    • 3
  • Guo Qun Zhao
    • 1
  • Si Zhang
    • 1
  • Jun Lu
    • 1
  • Shi Ru Jia
    • 4
  1. 1.Research Center for Fermentation Engineering of Hebei, College of Bioscience and BioengineeringHebei University of Science and TechnologyShijiazhangPR China
  2. 2.LibraryHebei University of Science and TechnologyShijiazhangPR China
  3. 3.Labour UnionHebei University of Science and TechnologyShijiazhangPR China
  4. 4.Key Laboratory of Industry Microbiology, Ministry of EducationTianjin University of Science and Technology, Tianjin Economic-Technological Development AreaTianjinPR China

Personalised recommendations