Molecules and Cells

, Volume 35, Issue 3, pp 210–218

Saccharomyces cerevisiae KNU5377 stress response during high-temperature ethanol fermentation

Authors

  • Il-Sup Kim
    • Advanced Bio-resource Research CenterKyungpook National University
  • Young-Saeng Kim
    • Department of BiologyKyungpook National University
  • Hyun Kim
    • Department of MicrobiologyKyungpook National University
  • Ingnyol Jin
    • Department of MicrobiologyKyungpook National University
    • Advanced Bio-resource Research CenterKyungpook National University
    • Department of BiologyKyungpook National University
Article

DOI: 10.1007/s10059-013-2258-0

Cite this article as:
Kim, I., Kim, Y., Kim, H. et al. Mol Cells (2013) 35: 210. doi:10.1007/s10059-013-2258-0

Abstract

Fuel ethanol production is far more costly to produce than fossil fuels. There are a number of approaches to costeffective fuel ethanol production from biomass. We characterized stress response of thermotolerant Saccharomyces cerevisiae KNU5377 during glucose-based batch fermentation at high temperature (40°C). S. cerevisiae KNU5377 (KNU5377) transcription factors (Hsf1, Msn2/4, and Yap1), metabolic enzymes (hexokinase, glyceraldehyde-3-phosphate dehydrogenase, glucose-6-phosphate dehydrogenase, isocitrate dehydrogenase, and alcohol dehydrogenase), antioxidant enzymes (thioredoxin 3, thioredoxin reductase, and porin), and molecular chaperones and its cofactors (Hsp104, Hsp82, Hsp60, Hsp42, Hsp30, Hsp26, Cpr1, Sti1, and Zpr1) are upregulated during fermentation, in comparison to S. cerevisiae S288C (S288C). Expression of glyceraldehyde-3-phosphate dehydrogenase increased significantly in KNU5377 cells. In addition, cellular hydroperoxide and protein oxidation, particularly lipid peroxidation of triosephosphate isomerase, was lower in KNU5377 than in S288C. Thus, KNU5377 activates various cell rescue proteins through transcription activators, improving tolerance and increasing alcohol yield by rapidly responding to fermentation stress through redox homeostasis and proteostasis.

Keywords

cell rescue proteinhigh-temperature fermentationredox stateSaccharomyces cerevisiae KNU5377stress response
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© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2013