Molecules and Cells

, Volume 34, Issue 3, pp 279–287

Rhythmic oscillation of histone acetylation and methylation at the Arabidopsis central clock loci

Research Article

DOI: 10.1007/s10059-012-0103-5

Cite this article as:
Song, HR. & Noh, YS. Mol Cells (2012) 34: 279. doi:10.1007/s10059-012-0103-5

Abstract

Circadian clock genes are regulated by a transcriptional-translational feedback loop. In Arabidopsis, LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSO-CIATED 1 (CCA1) transcripts are highly expressed in the morning. Translated LHY and CCA1 proteins repress the expression of TIMING OF CAB EXPRESSION 1 (TOC1), which peaks in the evening. TOC1 protein induces expression of LHY and CCA1, forming a negative feedback loop which is believed to constitute the oscillatory mechanism of the clock. The rhythmic oscillation of mouse clock genes mPERIOD 1 (mPER1) and mPER2 has been correlated with regular alteration of chromatin structure through histone acetylation/deacetylation. However, little is known about the relationship between the transcriptional activity of Arabidopsis clock genes and their chromatin status. Here, we report that histone H3 acetylation (H3Ac) and H3 lysine 4 tri-methylation (H3K4me3) levels at LHY, CCA1, and TOC1 are positively correlated with the rhythmic transcript levels of these genes, whereas H3K36me2 level shows a negative correlation. Thus, our study suggests rhythmic transcription of Arabidopsis clock genes might be regulated by rhythmic histone modification, and it provides a platform for future identification of clock-controlling histone modifiers.

Keywords

Arabidopsis chromatin circadian clock circadian rhythm histone modification 

Copyright information

© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2012

Authors and Affiliations

  1. 1.School of Biological SciencesSeoul National UniversitySeoulKorea
  2. 2.Biosafety Research Team, Environmental Health Research DepartmentNational Institute of Environmental ResearchIncheonKorea
  3. 3.Plant Genomics and Breeding InstituteSeoul National UniversitySeoulKorea

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