Molecules and Cells

, Volume 32, Issue 1, pp 77–82

Regulation of vascular endothelial growth factor signaling by miR-200b

  • Young-Chul Choi
  • Sena Yoon
  • Yongsu Jeong
  • Jaeseung Yoon
  • Kwanghee Baek
Article

DOI: 10.1007/s10059-011-1042-2

Cite this article as:
Choi, YC., Yoon, S., Jeong, Y. et al. Mol Cells (2011) 32: 77. doi:10.1007/s10059-011-1042-2

Abstract

Vascular endothelial growth factor (VEGF) signaling plays an important role in angiogenesis. In the VEGF signaling pathway, the key components are VEGF and its receptors, Flt-1 and KDR. In this study, we show that transfection of synthetic miR-200b reduced protein levels of VEGF, Flt-1, and KDR. In A549 cells, miR-200b targeted the predicted binding sites in the 3′-untranslated region (3′-UTR) of VEGF, Flt-1, and KDR as revealed by a luciferase reporter assay. When transfected with miR-200b, the ability of HUVECs to form a capillary tube on Matrigel and VEGF-induced phosphorylation of ERK1/2 were significantly reduced. Taken together, these results suggest that miR-200b negatively regulates VEGF signaling by targeting VEGF and its receptors and that miR-200b may have therapeutic potential as an angiogenesis inhibitor.

Keywords

Flt-1KDRmicroRNAmiR-200bVEGFVEGF signaling

Copyright information

© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2011

Authors and Affiliations

  • Young-Chul Choi
    • 1
  • Sena Yoon
    • 1
  • Yongsu Jeong
    • 1
  • Jaeseung Yoon
    • 1
  • Kwanghee Baek
    • 1
  1. 1.Graduate School of BiotechnologyKyung Hee UniversityYonginKorea