Molecules and Cells

, 32:251

TGF-β1 stimulates mouse macrophages to express APRIL through Smad and p38MAPK/CREB pathways

  • Young-Saeng Jang
  • Jae-Hee Kim
  • Goo-Young Seo
  • Pyeung-Hyeun Kim
Article

DOI: 10.1007/s10059-011-1040-4

Cite this article as:
Jang, YS., Kim, JH., Seo, GY. et al. Mol Cells (2011) 32: 251. doi:10.1007/s10059-011-1040-4

Abstract

A proliferation-inducing ligand (APRIL), a new TNF family member, supports B-cell survival and tumor cell proliferation. APRIL is secreted as a soluble protein by macrophages, dendritic cells and activated T cells. However, factors involved in regulation of APRIL expression are as yet unknown. In this study, we investigated the effect of TGF-β1 on APRIL expression in P388D1, a mouse macrophage cell line. TGF-β1 induced APRIL mRNA expression in a time- and dose-dependent manner. One nanogram per milliliter of TGF-β1 was optimal and APRIL transcripts appeared as early as 3 h after stimulation. Based on our studies, which included overexpression of Smad3, DN-Smad3, and sh-Smad3, we found that Smad3 mediates APRIL transcription at least partially. Further, experiments using inhibitors revealed that p38MAPK and CREB are also involved in TGF-β1-induced APRIL expression. These results suggest that TGF-β1, through Smad3 and p38MAPK/CREB signaling pathways, stimulates APRIL expression in macrophages.

Keywords

APRIL CREB p38 MAPK Smad3/4 TGF-β1 

Copyright information

© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2011

Authors and Affiliations

  • Young-Saeng Jang
    • 1
  • Jae-Hee Kim
    • 1
  • Goo-Young Seo
    • 1
  • Pyeung-Hyeun Kim
    • 1
    • 2
  1. 1.Department of Molecular BioscienceCollege of Biomedical ScienceChuncheonKorea
  2. 2.Medical and Bio-Material Research CenterKangwon National UniversityChuncheonKorea