Molecules and Cells

, Volume 31, Issue 3, pp 255–259

The S-nitrosylation of glyceraldehyde-3-phosphate dehydrogenase 2 is reduced by interaction with glutathione peroxidase 3 in Saccharomyces cerevisiae

  • Phil Young Lee
  • Kwang-Hee Bae
  • Dae Gwin Jeong
  • Seung-Wook Chi
  • Jeong Hee Moon
  • Seongman Kang
  • Sayeon Cho
  • Sang Chul Lee
  • Byoung Chul Park
  • Sung Goo Park
Article

DOI: 10.1007/s10059-011-0029-3

Cite this article as:
Lee, P.Y., Bae, KH., Jeong, D.G. et al. Mol Cells (2011) 31: 255. doi:10.1007/s10059-011-0029-3

Abstract

Glutathione peroxidases (Gpxs) are the key anti-oxidant enzymes found in Saccharomyces cerevisiae. Among the three Gpx isoforms, glutathione peroxidase 3 (Gpx3) is ubiquitously expressed and modulates the activities of redox-sensitive thiol proteins involved in various biological reactions. By using a proteomic approach, glyceraldehyde-3-phosphate dehydrogenase 2 (GAPDH2; EC 1.2.1.12) was found as a candidate protein for interaction with Gpx3. GAPDH, a key enzyme in glycolysis, is a multi-functional protein with multiple intracellular localizations and diverse activities. To validate the interaction between Gpx3 and GAPDH2, immunoprecipitation and a pull-down assay were carried out. The results clearly showed that GAPDH2 interacts with Gpx3 through its carboxyl-terminal domain both in vitro and in vivo. Additionally, Gpx3 helps to reduce the S-nitrosylation of GAPDH upon nitric oxide (NO) stress; this subsequently increases cellular viability. On the basis of our findings, we suggest that Gpx3 protects GAPDH from NO stress and thereby contributes to the maintenance of homeostasis during exposure to NO stress.

Keywords

ApoptosisGAPDHglutathione peroxidase 3NitosylationNO stress

Copyright information

© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2011

Authors and Affiliations

  • Phil Young Lee
    • 1
    • 2
  • Kwang-Hee Bae
    • 1
  • Dae Gwin Jeong
    • 1
  • Seung-Wook Chi
    • 1
  • Jeong Hee Moon
    • 1
  • Seongman Kang
    • 2
  • Sayeon Cho
    • 3
  • Sang Chul Lee
    • 1
  • Byoung Chul Park
    • 1
  • Sung Goo Park
    • 1
  1. 1.Medical Proteomics Research CenterKorea Research Institute of Bioscience and BiotechnologyDaejeonKorea
  2. 2.School of BiotechnologyKorea UniversitySeoulKorea
  3. 3.College of PharmacyChung-Ang UniversitySeoulKorea