Molecules and Cells

, Volume 27, Issue 1, pp 119–123

Identification and molecular characterization of PERV Gamma1 long terminal repeats

  • Jae-Won Huh
  • Dae-Soo Kim
  • Hong-Seok Ha
  • Kung Ahn
  • Kyu-Tae Chang
  • Byung-Wook Cho
  • Heui-Soo Kim
Communication

DOI: 10.1007/s10059-009-0013-3

Cite this article as:
Huh, JW., Kim, DS., Ha, HS. et al. Mol Cells (2009) 27: 119. doi:10.1007/s10059-009-0013-3

Abstract

Porcine endogenous retroviruses (PERVs) gamma1 in the pig genome have the potential to act as harmful factors in xenotransplantation (pig-to-human). Long terminal repeats (LTRs) are known to be strong promoter elements that could control the transcription activity of PERV elements and the adjacent functional genes. To investigate the transcribed PERV gamma1 LTR elements in pig tissues, bioin-formatic and experimental approaches were conducted. Using RT-PCR amplification and sequencing approaches, 69 different transcribed LTR elements were identified. And 69 LTR elements could be divided into six groups (15 subgroups) by internal variation including tandem repeated sequences, insertion and deletion (INDEL). Remarkably, all internal variations were indentified in U3 region of LTR elements. Taken together, the identification and characterization of various PERV LTR transcripts allow us to extend our knowledge of PERV and its transcriptional study.

Keywords

bioinformaticsLTR elementPERVPigxenotransplantation

Copyright information

© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2009

Authors and Affiliations

  • Jae-Won Huh
    • 1
    • 2
  • Dae-Soo Kim
    • 1
    • 3
  • Hong-Seok Ha
    • 1
  • Kung Ahn
    • 1
  • Kyu-Tae Chang
    • 2
  • Byung-Wook Cho
    • 4
  • Heui-Soo Kim
    • 1
  1. 1.Division of Biological Sciences, College of Natural SciencesPusan National UniversityBusanKorea
  2. 2.National Primate Research CenterKorea Research Institute of Bioscience and BiotechnologyChungbukKorea
  3. 3.Korean BioInformation CenterKorea Research Institute of Bioscience and BiotechnologyDaejeonKorea
  4. 4.Department of Animal Science, College of Life SciencesPusan National UniversityMiryangKorea