Implantation study of small-caliber “biotube” vascular grafts in a rat model
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We developed autologous vascular grafts, called “biotubes,” by simple and safe in-body tissue architecture technology, which is a practical concept of regenerative medicine, without using special sterile conditions or complicated in vitro cell treatment processes. In this study, biotubes of extremely small caliber were first auto-implanted to rat abdominal aortas. Biotubes were prepared by placing silicone rods (outer diameter 1.5 mm, length 30 mm) used as a mold into dorsal subcutaneous pouches in rats for 4 weeks. After argatroban coating, the obtained biotubes were auto-implanted to abdominal aortas (n = 6) by end-to-end anastomosis using a custom-designed sutureless vascular connecting system under microscopic guidance. Graft status was evaluated by contrast-free time-of-flight magnetic resonance angiography (TOF-MRA). All grafts were harvested at 12 weeks after implantation. The patency rate was 66.7 % (4/6). MRA showed little stenosis and no aneurysmal dilation in all biotubes. The original biotube had wall thickness of about 56.2 ± 26.5 μm at the middle portion and mainly random and sparse collagen fibers and fibroblasts. After implantation, the wall thickness was 235.8 ± 24.8 μm. In addition, native-like vascular structure was regenerated, which included (1) a completely endothelialized luminal surface, (2) a mesh-like elastin fiber network, and (3) regular circumferential orientation of collagen fibers and α-SMA positive cells. Biotubes could be used as small-caliber vascular prostheses that greatly facilitate the healing process and exhibit excellent biocompatibility in vascular regenerative medicine.
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- Implantation study of small-caliber “biotube” vascular grafts in a rat model
Journal of Artificial Organs
Volume 16, Issue 1 , pp 59-65
- Cover Date
- Print ISSN
- Online ISSN
- Springer Japan
- Additional Links
- Vascular grafts
- Autologous tissue
- In vivo tissue engineering
- Connective tissue
- Author Affiliations
- 1. Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka, 565-8565, Japan
- 2. Department of Cardiovascular Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan
- 3. Department of Pathology, National Cerebral and Cardiovascular Center, Osaka, Japan
- 4. Department of Biomedical Imaging, National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan
- 5. Department of Medical Physics and Engineering, Division of Health Sciences, Graduate School of Medicine, Osaka University, Osaka, Japan