Journal of Artificial Organs

, Volume 9, Issue 3, pp 179–184

Changes in expression of genes related to cell proliferation in human mesenchymal stem cells during in vitro culture in comparison with cancer cells

Authors

    • Division of Medical DevicesNational Institute of Health Sciences
  • Tomomi Ito
    • Division of Medical DevicesNational Institute of Health Sciences
  • Toshie Tsuchiya
    • Division of Medical DevicesNational Institute of Health Sciences
ORIGINAL ARTICLE

DOI: 10.1007/s10047-006-0338-z

Cite this article as:
Sawada, R., Ito, T. & Tsuchiya, T. J Artif Organs (2006) 9: 179. doi:10.1007/s10047-006-0338-z

Abstract

We investigated the expression levels of several genes related to cell proliferation in human mesenchymal stem cells (hMSCs) during in vitro culture for use in clinical applications. In this study, we focused on the relationship between hMSC proliferation and transforming growth factor β (TGFβ) signaling during in vitro culture. The proliferation rate of hMSCs gradually decreased and marked changes in hMSC morphology were not observed in 3 months of in vitro culture. The mRNA expressions of TGFβ1, TGFβ2, and TGFβ receptor type I (TGFβRI) in hMSCs increased with the length of cell culture. There had been no change in the TGFβ3, TGFβRII, and TGFβRIII mRNA expressions by the 12th passage from the primary culture (at about 3 months). The mRNA expression of Smad3 increased, but those of c-myc and nucleostemin decreased with the length of hMSC in vitro culture. In addition, the expression profiles of the genes that regulate cellular proliferation in hMSCs were significantly different from those of cancer cells. In conclusion, hMSCs derived from bone marrow seldom underwent spontaneous transformation during 1–2 months of in vitro culture for use in clinical applications. In hMSCs as well as in epithelial cells, growth might be controlled by the TGFβ family signaling.

Key words

Stem cellsCell proliferationTGFβ signalingTGFβ receptors

Copyright information

© The Japanese Society for Artificial Organs 2006