Optical Review

, Volume 16, Issue 2, pp 216–221

Influence of groove depth and surface profile on fluorescence enhancement by grating-coupled surface plasmon resonance

  • Hironobu Hori
  • Keiko Tawa
  • Kenji Kintaka
  • Junji Nishii
  • Yoshiro Tatsu
Regular Papers

DOI: 10.1007/s10043-009-0040-5

Cite this article as:
Hori, H., Tawa, K., Kintaka, K. et al. OPT REV (2009) 16: 216. doi:10.1007/s10043-009-0040-5

Abstract

The fluorescence intensity of a sample placed on a metal grating pattern is enhanced due to excitation by the electric field of the grating-coupled surface plasmon resonance (GC-SPR). The dependence of the enhancement on groove depth and surface profile was studied with the aim of improving the sensitivity of fluorescence detection. The enhancement was found to depend on the groove depth, with intensity most enhanced on grating substrate of about 20 nm depth, which produced an intensity about 30 times greater than that on a flat borosilicate glass substrate. Rigorous coupled wave analysis calculation showed that the shape of the groove influenced GC-SPR, suggesting that controlling not only the depth but also the shape of the grating surface profile can be an important factor in improving the sensitivity of detection by fluorescence microscopy.

Keywords

fluorescencemicroscopysurface plasmon resonancegrating structuresensitivity of fluorescence detection

Copyright information

© The Optical Society of Japan 2009

Authors and Affiliations

  • Hironobu Hori
    • 2
  • Keiko Tawa
    • 2
  • Kenji Kintaka
    • 1
  • Junji Nishii
    • 1
  • Yoshiro Tatsu
    • 2
  1. 1.Photonics Research InstituteNational Institute of Advanced Industrial Science and TechnologyIkeda, OsakaJapan
  2. 2.Research Institute for Cell EngineeringNational Institute of Advanced Industrial Science and TechnologyIkeda, OsakaJapan