Finding of Optimal Calcium Ion Probes for Fluorescence Lifetime Measurement
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- Yoshiki, K., Azuma, H., Yoshioka, K. et al. OPT REV (2005) 12: 415. doi:10.1007/s10043-005-0415-1
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We have investigated the fluorescence lifetime properties of 8 calcium ion probes, calcium-green-1, calcium green-2, calcium green-5N, calcium orange, oregon green 488 BAPTA-6F, fluo-3, fluo-4, and fluo-5N. We found that the decay time of calcium green-5N varied more sensitively with calcium concentration than calcium green-1 which was known to be a highly sensitive probe. We have also found that the center of observable range of calcium concentration by fluorescence lifetime measurement is lower than that by fluorescence intensity measurement.