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Direct electrochemistry and electrochemical biosensing of glucose oxidase based on CdSe@CdS quantum dots and MWNT-modified electrode

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Abstract

The direct electron transfer of glucose oxidase (GOx) was achieved based on the immobilization of CdSe@CdS quantum dots on glassy carbon electrode by multi-wall carbon nanotubes (MWNTs)-chitosan (Chit) film. The immobilized GOx displayed a pair of well-defined and reversible redox peaks with a formal potential (E θ’) of −0.459 V (versus Ag/AgCl) in 0.1 M pH 7.0 phosphate buffer solution. The apparent heterogeneous electron transfer rate constants (k s) of GOx confined in MWNTs-Chit/CdSe@CdS membrane were evaluated as 1.56 s−1 according to Laviron's equation. The surface concentration (Γ*) of the electroactive GOx in the MWNTs-Chit film was estimated to be (6.52 ± 0.01) × 10−11 mol cm−2. Meanwhile, the catalytic ability of GOx toward the oxidation of glucose was studied. Its apparent Michaelis–Menten constant for glucose was 0.46 ± 0.01 mM, showing a good affinity. The linear range for glucose determination was from 1.6 × 10−4 to 5.6 × 10−3 M with a relatively high sensitivity of 31.13 ± 0.02 μA mM−1 cm−2 and a detection limit of 2.5 × 10−5 M (S/N=3).

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Nos. 21175115), the Natural Science Foundation of Fujian province in China (2012J05031), the Zhangzhou Normal University scientific research projects (NO. SJ1117), and the Innovation Base Foundation for Graduate Students Education of Fujian Province.

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Authors and Affiliations

  1. Chemistry Department of Zhangzhou Normal university, Fujian, Zhangzhou, 363000, People’s Republic of China

    Fuying Huang, Fei Wang, Shuqing Feng, Yuanjun Li, Shunxing Li & Yancai Li

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  1. Fuying Huang
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  2. Fei Wang
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  3. Shuqing Feng
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  4. Yuanjun Li
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Correspondence to Yancai Li.

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Huang, F., Wang, F., Feng, S. et al. Direct electrochemistry and electrochemical biosensing of glucose oxidase based on CdSe@CdS quantum dots and MWNT-modified electrode. J Solid State Electrochem 17, 1295–1301 (2013). https://doi.org/10.1007/s10008-012-1986-y

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  • DOI: https://doi.org/10.1007/s10008-012-1986-y

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