Medical Electron Microscopy

, Volume 33, Issue 2, pp 74–81

Immunohistochemical localization of mitogen-activated protein kinase (MAPK) family and morphological changes in rat heart after ischemia-reperfusion injury

  • Z. Naito
  • Mitsuhiro Kudo
  • Guang Xu
  • Ryutaro Nishigaki
  • Munehiro Yokoyama
  • Nobutaka Yamada
  • Goro Asano
ORIGINAL PAPER

DOI: 10.1007/s007950070005

Cite this article as:
Naito, Z., Kudo, M., Xu, G. et al. Med Electron Microsc (2000) 33: 74. doi:10.1007/s007950070005

Abstract

The mitogen-activated protein kinase (MAPK) family is considered to be activated by stress, but the role of the MAPK family is still unknown in cardiac pathology. In the present study, not only the localization of MAPKs such as the extracellular responsive kinase (ERK), c-jun N-terminal kinase (JNK), and p38 MAPK (p38), but also ultrastructural changes were investigated in the ischemia-reperfusion model of Wistar rats. At 5, 10, 30, 60, and 180 min reperfusion after 30 min ischemia by occluding the coronary artery, the expression of these MAPKs was increased in blood vessels and cardiomyocytes by Western blotting and immunohistochemical methods. In addition, after ischemia reperfusion, various ultrastructural changes such as decreased glycogen granules, mitochondrial swelling, and myolysis were observed in the blood vessels and cardiomyocytes. These results suggest that protein kinases may regulate numerous biological processes, including the regulation of contraction and ion transport.

Key words Mitogen-activated protein kinase (MAPK) Ischemia reperfusion injury Heart Ultrastructure Immunohistochemistry 

Copyright information

© The Clinical Electron Microscopy Society of Japan 2000

Authors and Affiliations

  • Z. Naito
    • 1
  • Mitsuhiro Kudo
    • 1
  • Guang Xu
    • 1
  • Ryutaro Nishigaki
    • 1
  • Munehiro Yokoyama
    • 1
  • Nobutaka Yamada
    • 1
  • Goro Asano
    • 1
  1. 1.Department of Pathology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan Tel. +81-3-3822-2131; Fax +81-3-5685-3067 e-mail: asano/patho2@nms.ac.jpJP