Medical Electron Microscopy

, Volume 32, Issue 1, pp 11–19

Occludin modulates organization of perijunctional circumferential actin in rat endothelial cells

Authors

  • Shin-ichi Atsumi
    • Department of Pathology, Sapporo Medical University School of Medicine, S1 W17, Chuo-ku, Sapporo 060, Japan Tel. +81-11-611-2111; Fax +81-11-613-5665 e-mail: yasokoki@sapmed.ac.jp
  • Y. Kokai
    • Department of Pathology, Sapporo Medical University School of Medicine, S1 W17, Chuo-ku, Sapporo 060, Japan Tel. +81-11-611-2111; Fax +81-11-613-5665 e-mail: yasokoki@sapmed.ac.jp
  • Hirotoshi Tobioka
    • Department of Pathology, Sapporo Medical University School of Medicine, S1 W17, Chuo-ku, Sapporo 060, Japan Tel. +81-11-611-2111; Fax +81-11-613-5665 e-mail: yasokoki@sapmed.ac.jp
  • Kazuhide Kuwahara
    • Department of Pathology, Sapporo Medical University School of Medicine, S1 W17, Chuo-ku, Sapporo 060, Japan Tel. +81-11-611-2111; Fax +81-11-613-5665 e-mail: yasokoki@sapmed.ac.jp
  • Hiroki Kuwabara
    • Department of Pathology, Sapporo Medical University School of Medicine, S1 W17, Chuo-ku, Sapporo 060, Japan Tel. +81-11-611-2111; Fax +81-11-613-5665 e-mail: yasokoki@sapmed.ac.jp
  • Yasunari Takakuwa
    • Department of Pathology, Sapporo Medical University School of Medicine, S1 W17, Chuo-ku, Sapporo 060, Japan Tel. +81-11-611-2111; Fax +81-11-613-5665 e-mail: yasokoki@sapmed.ac.jp
  • Ken-ichi Sasaki
    • Department of Pathology, Sapporo Medical University School of Medicine, S1 W17, Chuo-ku, Sapporo 060, Japan Tel. +81-11-611-2111; Fax +81-11-613-5665 e-mail: yasokoki@sapmed.ac.jp
  • Norimasa Sawada
    • Department of Pathology, Sapporo Medical University School of Medicine, S1 W17, Chuo-ku, Sapporo 060, Japan Tel. +81-11-611-2111; Fax +81-11-613-5665 e-mail: yasokoki@sapmed.ac.jp
  • Toshihiro Mitaka
    • Department of Pathology, Cancer Research Institute, Sapporo Medical University School of Medicine, Sapporo, Japan
  • Yohichi Mochizuki
    • Department of Pathology, Cancer Research Institute, Sapporo Medical University School of Medicine, Sapporo, Japan
  • Kohzoh Imai
    • Department of Internal Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan
  • Michio Mori
    • Department of Pathology, Sapporo Medical University School of Medicine, S1 W17, Chuo-ku, Sapporo 060, Japan Tel. +81-11-611-2111; Fax +81-11-613-5665 e-mail: yasokoki@sapmed.ac.jp
ORIGINAL PAPER

DOI: 10.1007/s007950050003

Cite this article as:
Atsumi, S., Kokai, Y., Tobioka, H. et al. Med Electron Microsc (1999) 32: 11. doi:10.1007/s007950050003

Abstract

The tight junction is not a constitutional junctional apparatus in endothelial cells, but develops in a particular lineage of endothelia, such as the capillary endothelia in the brain and retina, and thus is considered to be pivotal for the maintenance of the blood–tissue barrier. Occludin is an integral membrane component of tight junctions, but the role of occludin in the endothelial cell function remains to be elucidated. We have cloned and transfected rat full-length occludin cDNA into a rat endothelial cell line (RLE) that expressed only a trace amount of occludin with no fine circumferential actin bundles at the cell border in native conditions. Occludin was expressed at the cell border of RLE cells, and circumferential fine actin bundles developed in close relation to the sites of occludin localization. Even under subconfluent culture conditions, fine circumferential actin bundles were formed at the sites where occludin-positive cell–cell contact was achieved. In immunoelectron microscopy, occludin was localized at distinct areas of the plasma membrane, always in association with the cytoplasmic actin filaments. On the other hand, actin bundles were not seen in occludin-negative juxtaposing plasma membranes. Collectively, these data strongly suggested a possible determinant function of occludin for the organization of actin in endothelial cells.

Key words EndothelOccludinActin

Copyright information

© The Clinical Electron Microscopy Society of Japan 1999