Medical Molecular Morphology

, 42:204

Expression and localization of tricellulin in human nasal epithelial cells in vivo and in vitro

  • Tsuyoshi Ohkuni
  • Takashi Kojima
  • Noriko Ogasawara
  • Tomoyuki Masaki
  • Takafumi Ninomiya
  • Shin Kikuchi
  • Mitsuru Go
  • Ken-ichi Takano
  • Tetsuo Himi
  • Norimasa Sawada
Original Paper

DOI: 10.1007/s00795-009-0470-y

Cite this article as:
Ohkuni, T., Kojima, T., Ogasawara, N. et al. Med Mol Morphol (2009) 42: 204. doi:10.1007/s00795-009-0470-y

Abstract

Tricellulin was identified as the first marker of the tricellular tight junction, which forms at the meeting points of three cells, and it is required for the maintenance of the transepithelial barrier. Although it is also considered to be important for the mucosal barrier of the upper respiratory tract, little is known about its expression and localization. In the present study, we examined the expression and localization of tricellulin in normal human nasal epithelial cells in vivo and in vitro, especially using primary cultures and telomerase reverse transcriptase (hTERT)-transfected cells. In human nasal epithelial cells in vivo and in vitro, mRNA and protein of tricellulin were detected. It was localized not only at tricellular contacts but also at bicellular borders, and in part colocalized with occludin. In human nasal epithelium, by immunoelectron microscopy analysis, tricellulin-associated gold particles were observed around the junction-like structure of the uppermost region. By treatment with 10% fetal bovine serum (FBS), expression of tricellulin mRNA was weakly increased, whereas that of bicellular tight junction molecules was strongly increased, in real-time PCR. These results suggest that tricellulin is stably expressed in human nasal epithelial cells and may play an important role for the sealing of the corner at tricellular contacts to prevent infiltration by various inhaled viruses and antigens.

Key words

Tight junctionsTricellulinHuman nasal epithelial cells

Copyright information

© The Japanese Society for Clinical Molecular Morphology 2009

Authors and Affiliations

  • Tsuyoshi Ohkuni
    • 1
    • 2
  • Takashi Kojima
    • 2
  • Noriko Ogasawara
    • 1
    • 2
  • Tomoyuki Masaki
    • 1
    • 2
  • Takafumi Ninomiya
    • 3
  • Shin Kikuchi
    • 3
  • Mitsuru Go
    • 1
  • Ken-ichi Takano
    • 1
  • Tetsuo Himi
    • 1
  • Norimasa Sawada
    • 2
  1. 1.Department of OtolaryngologySapporo Medical University School of MedicineSapporoJapan
  2. 2.Department of PathologySapporo Medical University School of MedicineSapporoJapan
  3. 3.Department of AnatomySapporo Medical University School of MedicineSapporoJapan