Medical Electron Microscopy

, Volume 37, Issue 3, pp 188–192

K-ras mutation may promote carcinogenesis of endometriosis leading to ovarian clear cell carcinoma

  • Junko Otsuka
  • Tsuyoshi Okuda
  • Akihiko Sekizawa
  • Satoshi Amemiya
  • Hiroshi Saito
  • Takashi Okai
  • Miki Kushima
  • Tetsuhiko Tachikawa
ORIGINAL PAPER

DOI: 10.1007/s00795-004-0252-5

Cite this article as:
Otsuka, J., Okuda, T., Sekizawa, A. et al. Med Electron Microsc (2004) 37: 188. doi:10.1007/s00795-004-0252-5

Abstract

Endometriosis shares some features characteristic of malignancy; however, it remains unclear whether endometriosis is a precursor to malignant disease. The objective is to determine the genetic relationship between endometriosis and ovarian clear cell carcinoma (OCCA). Among 37 Japanese patients with OCCA who underwent primary surgery at Showa University Hospital between 1987 and 1999, K-ras mutations were detected in 6. Three of these patients had ectopic endometrial tissue adjacent to the site of carcinoma. These cases demonstrated areas of endometriosis and areas of OCCA bordered by atypical endometriosis. We retrieved cells from regions of endometriosis and atypical endometriosis, as well as OCCA cells, by laser microdissection in each case. K-ras mutations were analyzed in each specimen dissected. DNA analysis of each region revealed that K-ras mutations were detectable in OCCA but not in endometriosis or atypical endometriosis. It is thought that a number of genetic alterations are involved in malignant transformation. It is possible that K-ras mutations are associated with malignant transformation of atypical endometriosis into OCCA, although further research is needed to define this mechanism.

Key words

Ovarian endometrioid cancerAtypical endometriosisK-ras mutationLaser microdissection

Copyright information

© The Clinical Electron Microscopy Society of Japan 2004

Authors and Affiliations

  • Junko Otsuka
    • 1
  • Tsuyoshi Okuda
    • 1
  • Akihiko Sekizawa
    • 1
  • Satoshi Amemiya
    • 1
  • Hiroshi Saito
    • 1
  • Takashi Okai
    • 1
  • Miki Kushima
    • 2
  • Tetsuhiko Tachikawa
    • 3
  1. 1.Department of Obstetrics and GynecologyShowa University School of MedicineTokyoJapan
  2. 2.Department of Hospital PathologyShowa University School of MedicineTokyoJapan
  3. 3.Department of Oral PathologyShowa University School of DentistryTokyoJapan