ORIGINAL PAPER

Extremophiles

, Volume 4, Issue 6, pp 373-376

Direct proof for the presence and expression of two 5% different 16S rRNA genes in individual cells of Haloarcula marismortui

  • Gudrun AmannAffiliated withLehrstuhl für Mikrobiologie, Universität Regensburg, 93053 Regensburg, Germany Tel. +49-941-9433161; Fax +49-941-9432403 e-mail: karl.stetter@biologie.uni-regensburg.de
  • , K. O. StetterAffiliated withLehrstuhl für Mikrobiologie, Universität Regensburg, 93053 Regensburg, Germany Tel. +49-941-9433161; Fax +49-941-9432403 e-mail: karl.stetter@biologie.uni-regensburg.de
  • , Enric Llobet-BrossaAffiliated withMax-Planck Institute for Marine Microbiology, Bremen, Germany
  • , Rudolf AmannAffiliated withMax-Planck Institute for Marine Microbiology, Bremen, Germany
  • , Josefa AntónAffiliated withMax-Planck Institute for Marine Microbiology, Bremen, Germany

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Abstract

A single-cell-derived pure culture of Haloarcula marismortui was obtained using the optical tweezers technique. Analysis of the 16S rRNA genes unambiguously demonstrated two 5% different 16S rRNA sequences. In addition, expression of both operons in the same cell was shown by fluorescence in situ hybridization with operon-specific probes.

Key words 16S rDNA heterogeneity Archaea Haloarcula marismortui Fluorescence in situ hybridization