Direct proof for the presence and expression of two 5% different 16S rRNA genes in individual cells of Haloarcula marismortui
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- Amann, G., Stetter, K., Llobet-Brossa, E. et al. Extremophiles (2000) 4: 373. doi:10.1007/s007920070007
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A single-cell-derived pure culture of Haloarcula marismortui was obtained using the optical tweezers technique. Analysis of the 16S rRNA genes unambiguously demonstrated two 5% different 16S rRNA sequences. In addition, expression of both operons in the same cell was shown by fluorescence in situ hybridization with operon-specific probes.