ORIGINAL PAPER

Extremophiles

, Volume 3, Issue 4, pp 235-238

First online:

Characterization and radiation resistance of new isolates of Rubrobacter radiotolerans and Rubrobacter xylanophilus

  • A. C. FerreiraAffiliated withDepartamento de Bioquímica Universidade de Coimbra, 3000 Coimbra, Portugal Tel. +351-39-824024; Fax +351-39-26798 e-mail. milton@cygnus.ci.uc.pt
  • , M. F. NobreAffiliated withDepartamento de Zoologia, Universidade de Coimbra, 3004-517 Coimbra, Portugal
  • , E. MooreAffiliated withBereich Mikrobiologie, Gesellschaft fur Biotechnologische Forschung, Braunschweig, Germany
  • , F. A. RaineyAffiliated withDepartment of Biological Sciences, Louisiana State University, Baton Rouge, LA, USA
  • , J. R. BattistaAffiliated withDepartment of Biological Sciences, Louisiana State University, Baton Rouge, LA, USA
  • , M.S. da CostaAffiliated withDepartamento de Bioquímica Universidade de Coimbra, 3000 Coimbra, Portugal Tel. +351-39-824024; Fax +351-39-26798 e-mail. milton@cygnus.ci.uc.pt

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Abstract

In this study we characterized new strains of the slightly thermophilic species Rubrobacter radiotolerans and the thermophilic species Rubrobacter xylanophilus, both of which were previously represented only by the type strains isolated, respectively, from Japan and the United Kingdom. The new isolates were recovered from two hot springs in central Portugal after gamma irradiation of water and biofilm samples. We assessed biochemical characteristics, performed DNA–DNA hybridization, and carried out 16S rDNA sequence analysis to demonstrate that the new Rubrobacter isolates belong to the species R. radiotolerans and R. xylanophilus. We also show for the first time that the strains of R. xylanophilus and other strains of R. radiotolerans are extremely gamma radiation resistant.

Key wordsRubrobacter radiotolerans Rubrobacter xylanophilus Radiation resistance Hot springs Distribution