Extremophiles

, Volume 3, Issue 3, pp 227–233

Genetic analysis of the chromosome of alkaliphilic Bacillus halodurans C-125

  • H. Takami
  • Yoshihiro Takaki
  • Kaoru Nakasone
  • Tokuki Sakiyama
  • G. Maeno
  • Rumie Sasaki
  • Chie Hirama
  • Fumie Fuji
  • Noriaki Masui

DOI: 10.1007/s007920050120

Cite this article as:
Takami, H., Takaki, Y., Nakasone, K. et al. Extremophiles (1999) 3: 227. doi:10.1007/s007920050120

Abstract

Seventeen Sse8387I linking clones isolated from the chromosome of Bacillus halodurans C-125 for the purpose of constructing a physical map were sequenced and analyzed by comparison with the BSORF database and the nonredundant protein databank. The orientations of Sse8387I or AscI linking clones serving to join adjacent fragments were determined by southern blot analysis using specific DNA probes. One-third of the open reading frames (ORFs) identified in the Sse8387I linking clones showed no significant similarity to any protein so far reported. The ORFs showing significant similarities to those of Bacillus subtilis were mapped in the chromosome of strain C-125, and the locations of the putative genes on the map were not well conserved between B. halodurans C-125 and B. subtilis.

Key words Alkaliphile Bacillus halodurans C-125 Genetic map Genome analysis 

Copyright information

© Springer-Verlag Tokyo 1999

Authors and Affiliations

  • H. Takami
    • 1
  • Yoshihiro Takaki
    • 1
  • Kaoru Nakasone
    • 1
  • Tokuki Sakiyama
    • 1
  • G. Maeno
    • 1
  • Rumie Sasaki
    • 1
  • Chie Hirama
    • 1
  • Fumie Fuji
    • 1
  • Noriaki Masui
    • 1
  1. 1.Deep-sea Microorganisms Research Group, Japan Marine Science and Technology Center, 2-15 Natsushima, Yokosuka 237-0061, Japan Tel. +81-468-67-3895; Fax +81-468-66-6364 e-mail: takamih@jamstec.go.jpJP