Extremophiles

, Volume 3, Issue 2, pp 97-102

First online:

Biodiversity in deep-sea sites located near the south part of Japan

  • H. TakamiAffiliated withDeep-Sea Microorganisms Research Group, Japan Marine Science and Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan Tel. +81-468-67-3895; Fax +81-468-66-6364 e-mail: takamih@jamstec.go.jp
  • , Kuniko KobataAffiliated withDeep-Sea Microorganisms Research Group, Japan Marine Science and Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan Tel. +81-468-67-3895; Fax +81-468-66-6364 e-mail: takamih@jamstec.go.jp
  • , Takahiko NagahamaAffiliated withDeep-Sea Microorganisms Research Group, Japan Marine Science and Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan Tel. +81-468-67-3895; Fax +81-468-66-6364 e-mail: takamih@jamstec.go.jp
  • , Hideki KobayashiAffiliated withDeep-Sea Microorganisms Research Group, Japan Marine Science and Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan Tel. +81-468-67-3895; Fax +81-468-66-6364 e-mail: takamih@jamstec.go.jp
  • , Akira InoueAffiliated withDeep-Sea Microorganisms Research Group, Japan Marine Science and Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan Tel. +81-468-67-3895; Fax +81-468-66-6364 e-mail: takamih@jamstec.go.jp
  • , Koki HorikoshiAffiliated withDeep-Sea Microorganisms Research Group, Japan Marine Science and Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan Tel. +81-468-67-3895; Fax +81-468-66-6364 e-mail: takamih@jamstec.go.jp

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Abstract

We obtained 100 isolates of bacteria from deep-sea mud samples collected at various depths (1050–10 897 m). Various types of bacteria such as alkaliphiles, thermophiles, psychrophiles, and halophiles were recovered on agar plates at a frequency of 0.8 × 102 to 2.3 × 104/g of dry sea mud. No acidophiles were recovered. These extremophilic bacteria were widely distributed, being detected at each deep-sea site, and the frequency of isolation of such extremophiles from the deep-sea mud was not directly influenced by the depth of the sampling sites. Phylogenetic analysis of deep-sea isolates based on 16S rDNA sequences revealed that a wide range of taxa were represented in the deep-sea environments. Growth patterns under high hydrostatic pressure were determined for the deep-sea isolates obtained in this study. No extremophilic strains isolated in this study showed growth at 60 MPa, although a few of the other isolates grew slightly at this hydrostatic pressure.

Key words Extremophiles Halophiles Psychrophiles Alkaliphiles Thermophiles Deep sea 16S ribosomal RNA Phylogenetic tree