Extremophiles

, Volume 1, Issue 1, pp 14–21

Pyrolobus fumarii, gen. and sp. nov., represents a novel group of archaea, extending the upper temperature limit for life to 113°C

  • Elisabeth Blöchl
  • Reinhard Rachel
  • Siegfried Burggraf
  • Doris Hafenbradl
  • Holger W. Jannasch
  • Karl O. Stetter
ORIGINAL PAPER

DOI: 10.1007/s007920050010

Cite this article as:
Blöchl, E., Rachel, R., Burggraf, S. et al. Extremophiles (1997) 1: 14. doi:10.1007/s007920050010

Abstract

A novel, irregular, coccoid-shaped archaeum was isolated from a hydrothermally heated black smoker wall at the TAG site at the Mid Atlantic Ridge (depth 3650 meters). It grew at between 90°C and 113°C (optimum 106°C) and pH 4.0–6.5 (optimum 5.5) and 1%–4% salt (optimum 1.7%). The organism was a facultatively aerobic obligate chemolithoautotroph gaining energy by H2-oxidation. Nitrate, S2O32–, and low concentrations of O2 (up to 0.3% v/v) served as electron acceptors, yielding NH+4, H2S, and H2O as end products, respectively. Growth was inhibited by acetate, pyruvate, glucose, starch, or sulfur. The new isolate was able to form colonies on plates (at 102°C) and to grow at a pressure of 25000 kPa (250 bar). Exponentially growing cultures survived a one-hour autoclaving at 121°C. The GC content was 53mol%. The core lipids consisted of glycerol–dialkyl glycerol tetraethers and traces of 2,3-di-O-phytanyl-sn-glycerol. The cell wall was composed of a surface layer of tetrameric protein complexes arranged on a p4-lattice (center-to-center distance 18.5nm). By its 16S rRNA sequence, the new isolate belonged to the Pyrodictiaceae. Based on its GC-content, DNA homology, S-layer composition, and metabolism, we describe here a new genus, which we name Pyrolobus (the "fire lobe"). The type species is Pyrolobus fumarii (type strain 1A; DSM).

Key words Archaea, Hyperthermophilic, Temperature, Hydrothermal, Taxonomy, Marine

Copyright information

© Springer-Verlag Tokyo 1997

Authors and Affiliations

  • Elisabeth Blöchl
    • 1
  • Reinhard Rachel
    • 1
  • Siegfried Burggraf
    • 1
  • Doris Hafenbradl
    • 1
  • Holger W. Jannasch
    • 2
  • Karl O. Stetter
    • 1
  1. 1.Lehrstuhl für Mikrobiologie und Archaeenzentrum, Universität Regensburg, Universitätsstraße 31, D-93053 Regensburg, Germany, Tel. +49-941-943-3160; Fax +49-941-943-2403; e-mail: karl.stetter@biologie.uni-regensburg.deDE
  2. 2.Department of Biology, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USAUS