Extremophiles

, Volume 12, Issue 1, pp 51–59

The effect of temperature on enzyme activity: new insights and their implications

  • Roy M. Daniel
  • Michael J. Danson
  • Robert Eisenthal
  • Charles K. Lee
  • Michelle E. Peterson
Review

DOI: 10.1007/s00792-007-0089-7

Cite this article as:
Daniel, R.M., Danson, M.J., Eisenthal, R. et al. Extremophiles (2008) 12: 51. doi:10.1007/s00792-007-0089-7

Abstract

The two established thermal properties of enzymes are their activation energy and their thermal stability. Arising from careful measurements of the thermal behaviour of enzymes, a new model, the Equilibrium Model, has been developed to explain more fully the effects of temperature on enzymes. The model describes the effect of temperature on enzyme activity in terms of a rapidly reversible active-inactive transition, in addition to an irreversible thermal inactivation. Two new thermal parameters, Teq and ΔHeq, describe the active–inactive transition, and enable a complete description of the effect of temperature on enzyme activity. We review here the Model itself, methods for the determination of Teq and ΔHeq, and the implications of the Model for the environmental adaptation and evolution of enzymes, and for biotechnology.

Keywords

Equilibrium ModelTemperatureEnzymeAdaptationEvolutionTemperature optimumStabilityDenaturationTeqΔHeq

Copyright information

© Springer 2007

Authors and Affiliations

  • Roy M. Daniel
    • 1
  • Michael J. Danson
    • 2
  • Robert Eisenthal
    • 3
  • Charles K. Lee
    • 1
  • Michelle E. Peterson
    • 1
  1. 1.Department of Biological SciencesUniversity of WaikatoHamiltonNew Zealand
  2. 2.Centre for Extremophile Research, Department of Biology and BiochemistryUniversity of BathBathUK
  3. 3.Department of Biology and BiochemistryUniversity of BathBathUK