Extremophiles

, Volume 11, Issue 4, pp 585–596

Structure-dependent relationships between growth temperature of prokaryotes and the amino acid frequency in their proteins

  • Gisle Sælensminde
  • Øyvind HalskauJr
  • Ronny Helland
  • Nils-Peder Willassen
  • Inge Jonassen
Original Paper

DOI: 10.1007/s00792-007-0072-3

Cite this article as:
Sælensminde, G., Halskau, Ø., Helland, R. et al. Extremophiles (2007) 11: 585. doi:10.1007/s00792-007-0072-3

Abstract

We studied the amino acid frequency and substitution patterns between homologues of prokaryotic species adapted to temperatures in the range 0–102°C, and found a significant temperature-dependent difference in frequency for many of the amino acids. This was particularly clear when we analysed the surface and core residues separately. The difference between the surface and the core is getting more pronounced in proteins adapted to warmer environments, with a more hydrophobic core, and more charged and long-chained amino acids on the surface of the proteins. We also see that mesophiles have a more similar amino acid composition to psychrophiles than to thermophiles, and that archea appears to have a slightly different pattern of substitutions than bacteria.

Keywords

ThermophilesPsychrophilesCold-adaptationStabilityAmino acid frequencyProtein structure

Abbreviations

OGT

Optimal growth temperature

SASA

Solvent accessible surface area

Supplementary material

Copyright information

© Springer 2007

Authors and Affiliations

  • Gisle Sælensminde
    • 1
    • 6
  • Øyvind HalskauJr
    • 2
  • Ronny Helland
    • 3
    • 4
  • Nils-Peder Willassen
    • 4
    • 5
  • Inge Jonassen
    • 1
    • 6
  1. 1.Computational Biology Unit (CBU), BCCSUniversity of BergenBergenNorway
  2. 2.Department of BiomedicineUniversity of BergenBergenNorway
  3. 3.Department of ChemistryUniversity of TromsøTromsøNorway
  4. 4.Norwegian Structural Biology CentreUniversity of TromsøTromsøNorway
  5. 5.Institute of Medical Biology, Faculty of MedicineUniversity of TromsøTromsøNorway
  6. 6.Department of InformaticsUniversity of BergenBergenNorway