Extremophiles

, Volume 10, Issue 5, pp 441–449

Phenol degradation by immobilized cold-adapted yeast strains of Cryptococcus terreus and Rhodotorula creatinivora

  • Irina Krallish
  • Svetlana Gonta
  • Ludmila Savenkova
  • Phillip Bergauer
  • Rosa Margesin
Original Paper

DOI: 10.1007/s00792-006-0517-0

Cite this article as:
Krallish, I., Gonta, S., Savenkova, L. et al. Extremophiles (2006) 10: 441. doi:10.1007/s00792-006-0517-0
  • 173 Downloads

Abstract

Three strains were isolated from hydrocarbon-polluted alpine habitats and were representatives of Cryptococcus terreus (strain PB4) and Rhodotorula creatinivora (strains PB7, PB12). All three strains synthesized and accumulated glycogen (both acid- and alkali-soluble) and trehalose during growth in complex medium containing glucose as carbon source and in minimal salt medium (MSM) with phenol as sole carbon and energy source. C. terreus strain PB4 showed a lower total accumulation level of storage compounds and a lower extracellular polysaccharides (EPS) production than the two R. creatinivora strains, PB7 and PB12. Biofilm formation and phenol degradation by yeast strains attached to solid carriers of zeolite or filter sand were studied at 10°C. Phenol degradation by immobilized yeast strains was always higher on zeolite compared with filter sand under normal osmotic growth conditions. The transfer of cells immobilized on both solid supports to a high osmotic environment decreased phenol degradation activity by all strains. However, both R. creatinivora PB7 and PB12 strains maintained higher ability to degrade phenol compared with C. terreus strain PB4, which almost completely lost its phenol degradation activity. Moreover, R. creatinivora strain PB7 showed the highest ability to form biofilm on both carriers under high osmotic conditions of cultivation.

Keywords

Yeasts Rhodotorula creatinivora Cryptococcus terreus Cold-adapted Phenol Biodegradation Storage compounds Extracellular polysaccharides Biofilm Immobilization 

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Irina Krallish
    • 1
  • Svetlana Gonta
    • 1
  • Ludmila Savenkova
    • 1
  • Phillip Bergauer
    • 2
  • Rosa Margesin
    • 2
  1. 1.Institute of Microbiology and BiotechnologyUniversity of Latvia RigaLatvia
  2. 2.Institute of MicrobiologyLeopold Franzens UniversityInnsbruckAustria

Personalised recommendations