, Volume 8, Issue 5, pp 351-356
Date: 27 May 2004

Genomic characterization of thermophilic Geobacillus species isolated from the deepest sea mud of the Mariana Trench

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Abstract

The thermophilic strains HTA426 and HTA462 isolated from the Mariana Trench were identified as Geobacillus kaustophilus and G. stearothermophilus, respectively, based on physiologic and phylogenetic analyses using 16S rDNA sequences and DNA–DNA relatedness. The genome size of HTA426 and HTA462 was estimated at 3.23–3.49 Mb and 3.7–4.49 Mb, respectively. The nucleotide sequences of three independent λ-phage inserts of G. stearothermophilus HTA462 have been determined. The organization of protein coding sequences (CDSs) in the two λ-phage inserts was found to differ from that in the contigs corresponding to each λ insert assembled by the shotgun clones of the G. kaustophilus HTA426 genome, although the CDS organization in another λ insert is identical to that in the HTA426 genome.

Communicated by K. Horikoshi