Original Paper

Extremophiles

, Volume 8, Issue 4, pp 317-323

First online:

The first evidence of anaerobic CO oxidation coupled with H2 production by a hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent

  • Tatyana G. SokolovaAffiliated withInstitute of Microbiology, Russian Academy of Sciences Email author 
  • , Christian JeanthonAffiliated withUMR 6539 Centre National de la Recherche Scientifique et Université de Bretagne Occidentale, Institut Universitaire Européen de la Mer
  • , Nadezhda A. KostrikinaAffiliated withInstitute of Microbiology, Russian Academy of Sciences
  • , Nikolai A. ChernyhAffiliated withInstitute of Microbiology, Russian Academy of Sciences
  • , Alexander V. LebedinskyAffiliated withInstitute of Microbiology, Russian Academy of Sciences
  • , Erko StackebrandtAffiliated withDSMZ, German Collection of Microorganisms and Cell Cultures
  • , Elizaveta A. Bonch-OsmolovskayaAffiliated withInstitute of Microbiology, Russian Academy of Sciences

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Abstract

From 24 samples of hydrothermal venting structures collected at the East Pacific Rise (13°N), 13 enrichments of coccoid cells were obtained which grew on CO, producing H2 and CO2 at 80°C. A hyperthermophilic archaeon capable of lithotrophic growth on CO coupled with equimolar production of H2 was isolated. Based on its 16S rRNA sequence analysis, this organism was affiliated with the genus Thermococcus. Other strains of Thermococcales species (Pyrococcus furiosus, Thermococcus peptonophilus, T. profundus, T. chitonophagus, T. stetteri, T. gorgonarius, T. litoralis, and T. pacificus) were shown to be unable to grow on CO. Searches in sequence databases failed to reveal deposited sequences of genes related to CO metabolism in Thermococcales. Our work provides the first evidence of anaerobic CO oxidation coupled with H2 production performed by an archaeon as well as the first documented case of lithotrophic growth of a Thermococcales representative.

Keywords

Anaerobic CO oxidation Deep-sea hot vents Hyperthermophilic archaea Thermococcus