Extremophiles

, Volume 7, Issue 4, pp 291–298

Piezophysiology of genome wide gene expression levels in the yeast Saccharomyces cerevisiae

  • Hitoshi Iwahashi
  • Hisayo Shimizu
  • Mine Odani
  • Yasuhiko Komatsu
Original Paper

DOI: 10.1007/s00792-003-0322-y

Cite this article as:
Iwahashi, H., Shimizu, H., Odani, M. et al. Extremophiles (2003) 7: 291. doi:10.1007/s00792-003-0322-y

Abstract

Hydrostatic pressure is one of the physical factors affecting cellular physiology. Hydrostatic pressure of a few hundred MPa decreases the viability of yeast cells, and pressure of a few tens MPa decreases the growth rate. To understand the effect of hydrostatic pressure, we employed yeast DNA microarrays and analyzed genome-wide gene-expression levels after the pressure treatment with 180 MPa (immediate) at 4°C and recovery incubation for 1 h and 40 MPa (16 h) at 4°C and recovery incubation for 1 h. The transcription of genes involved in energy metabolism, cell defense, and protein metabolism was significantly induced by the pressure treatment. Genome-wide expression profiles suggested that high pressure caused damage to cellular organelles, since the induced gene products were localized in the membrane structure and/or cellular organelles. Hierarchical clustering analysis suggested that the damage caused by the pressure was similar to that caused by detergents, oils, and freezing/thawing. We also estimated the contribution of induced genes to barotolerance using some strains that have the deletion in the corresponding genes.

Keywords

Barotolerance Gene expression Hydrostatic pressure Piezophysiology Saccharomyces cerevisiae Yeast 

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Hitoshi Iwahashi
    • 1
    • 2
  • Hisayo Shimizu
    • 2
  • Mine Odani
    • 2
  • Yasuhiko Komatsu
    • 2
  1. 1.Human Stress Signal Research CenterNational Institute of Advanced Industrial Science and TechnologyTsukuba 305-8566Japan
  2. 2.International Patent Organism DepositaryNational Institute of Advanced Industrial Science and TechnologyTsukuba 305-8566Japan

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