The fate and role of bone graft-derived cells after autologous tendon and bone transplantation into the bone tunnel
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Grafting bone between the tendon graft and the bone tunnel in anterior cruciate ligament reconstruction increases the mechanical strength of the tendon graft. However, the biological role of the bone graft is unclear. The purpose of this research was to elucidate the role of bone graft cells after autologous tendon graft into the bone tunnel with an autologous bone graft in green fluorescent protein (GFP) transgenic rats.
The Achilles tendons of Sprague-Dawley (SD) wild-type rats and bone of GFP rats were harvested and transplanted into bone tunnels drilled in the femurs at the knees of SD rats. The femurs were harvested at 1, 2, and 4 weeks after transplantation and histologically investigated using hematoxylin and eosin staining and immunostaining of heat shock protein 47 (HSP47), macrophages, and type I and type III collagens. Biomechanical tests were performed on the tendon graft 2 and 4 weeks after transplantation to evaluate the ultimate force to failure.
A small number of GFP-positive cells was seen in the tendon graft 2 weeks after transplantation. The cell count in the tendon graft was increased at 4 weeks after transplantation. HSP47-positive cells and macrophage-stained cells present in the tendon graft corresponded with the GFP-positive cells. By 2 weeks after transplantation, the relative areas of immunostained type I and III collagens in the tendon graft had declined significantly in the bone graft group compared to the control. The ultimate failure load in the bone graft group was higher than that in the control group at both 2 and 4 weeks after transplantation.
This research showed that, within 4 weeks of transplantation, bone graft cells migrate to the tendon graft, where they differentiate into cells involved in collagen production and macrophages. Bone graft cells may contribute to the early stage remodeling of tendon grafts.
- Rosenberg TD, Deffner KT. ACL reconstruction: semitendinosus tendon is the graft of choice. Orthopedics. 1997;20:396–8.
- Hara K, Arai Y, Ohta M, Minami G, Urade H, Hirai N, Watanabe N, Kubo T. A new double-bundle anterior cruciate ligament reconstruction using the posteromedial portal technique with hamstrings. Arthroscopy. 2005;21:1274.
- Muneta T, Koga H, Mochizuki T, Ju YJ, Hara K, Nimura A, Yagishita K, Sekiya I. A prospective randomized study of 4-strand semitendinosus tendon anterior cruciate ligament reconstruction comparing single-bundle and double-bundle techniques. Arthroscopy. 2007;23:618–28. CrossRef
- Yasuda K, Tanabe Y, Kondo E, Kitamura N, Tohyama H. Anatomic double-bundle anterior cruciate ligament reconstruction. Arthroscopy. 2010;26:21–34.
- Arnoczky SP, Tarvin GB, Marshall JL. Anterior cruciate ligament replacement using patellar tendon. An evaluation of graft revascularization in the dog. J Bone Joint Surg Am. 1982;64:217–24.
- Amiel D, Kleiner JB, Akeson WH. The natural history of the anterior cruciate ligament autograft of patellar tendon origin. Am J Sports Med. 1986;14:449–62. CrossRef
- Shino K, Kawasaki T, Hirose H, Gotoh I, Inoue M, Ono K. Replacement of the anterior cruciate ligament by an allogeneic tendon graft. An experimental study in the dog. J Bone Joint Surg. 1984;66:672–81.
- Blickenstaff KR, Grana WA, Egle D. Analysis of a semitendinosus autograft in a rabbit model. Am J Sports Med. 1997;25:554–9. CrossRef
- Okabe M, Ikawa M, Kominami K, Nakanishi T, Nishimune Y. ‘Green mice’ as a source of ubiquitous green cells. FEBS Lett. 1997;407:313–9. CrossRef
- Ito T, Suzuki A, Okabe M, Imai E, Hori M. Application of bone marrow-derived stem cells in experimental nephrology. Exp Nephrol. 2001;9:444–50. CrossRef
- Watanabe N, Takai S, Morita N, Kawata M, Hirasawa Y. A method of tracking donor cells after simulated autologous transplantation: a study using synovial cells of transgenic rats. Cell Tissue Res. 1999;298:519–25. CrossRef
- Oshima Y, Watanabe N, Matsuda K, Takenaka N, Kawata M, Takai S. Behavior of graft and host cells in underlying subchondral bone after transplantation of osteochondral autograft. Microsc Res Tech. 2002;58:19–24. CrossRef
- Kobayashi M, Watanabe N, Oshima Y, Kajikawa Y, Kawata M, Kubo T. The fate of host and graft cells in early healing of bone tunnel after tendon graft. Am J Sports Med. 2005;33:1892–7. CrossRef
- Tachiiri H, Morihara T, Iwata Y, Yoshida A, Kajikawa Y, Kida Y, Matsuda K, Fujiwara H, Kurokawa M, Kawata M, Kubo T. Characteristics of donor and host cells in the early remodeling process after transplant of Achilles tendon with and without live cells for the treatment of rotator cuff defect—what is the ideal graft for the treatment of massive rotator cuff defects? J Should Elbow Surg. 2010;19:891–8. CrossRef
- Dovan TT, Ritty T, Ditsios K, Silva MJ, Kusano N, Gelberman RH. Flexor digitorum profundus tendon to bone tunnel repair: a vascularization and histologic study in canines. J Hand Surg Am. 2005;30:246–57. CrossRef
- Arai Y, Hara K, Takahashi T, Urade H, Minami G, Takamiya H, Kubo T. Evaluation of the vascular status of autogenous hamstring tendon grafts after anterior cruciate ligament reconstruction in humans using magnetic resonance angiography. Knee Surg Sports Traumatol Arthrosc. 2008;16:342–7. CrossRef
- Yukata K, Matsui Y, Shukunami C, Takimoto A, Hirohashi N, Ohtani O, Kimura T, Hiraki Y, Yasui N. Differential expression of tenomodulin and chondromodulin-1 at the insertion site of the tendon reflects a phenotypic transition of the resident cells. Tissue Cell. 2010;42(2):116–20. CrossRef
- Howell SM, Roos P, Hull ML. Compaction of a bone dowel in the tibial tunnel improves the fixation stiffness of a soft tissue anterior cruciate ligament graft: an in vitro study in calf tibia. Am J Sports Med. 2005;33:719–25. CrossRef
- To JT, Howell SM, Hull ML. Contributions of femoral fixation methods to the stiffness of anterior cruciate ligament replacements at implantation. Arthroscopy. 1999;15:379–87. CrossRef
- Masuda H, Hosokawa N, Nagata K. Expression and localization of collagen-binding stress protein Hsp47 in mouse embryo development: comparison with types I and III collagen. Cell Stress Chaperones. 1998;3:256–64. CrossRef
- Nagata K. Expression and function of heat shock protein 47: a collagen-specific molecular chaperone in the endoplasmic reticulum. Matrix Biol. 1998;16:379–86. CrossRef
- Kawamura S, Ying L, Kim HJ, Dynybil C, Rodeo SA. Macrophages accumulate in the early phase of tendon–bone healing. J Orthop Res. 2005;23:1425–32.
- Hays PL, Kawamura S, Deng XH, Dagher E, Mithoefer K, Ying L, Rodeo SA. The role of macrophages in early healing of a tendon graft in a bone tunnel. J Bone Joint Surg Am. 2008;90:565–79. CrossRef
- Goradia VK, Rochat MC, Kida M, Grana WA. Natural history of a hamstring tendon autograft used for anterior cruciate ligament reconstruction in a sheep model. Am J Sports Med. 2000;28(1):40–6.
- Cypher TJ, Grossman JP. Biological principles of bone graft healing. J Foot Ankle Surg. 1996;35:413–7. CrossRef
- Kajikawa Y, Morihara T, Watanabe N, Sakamoto H, Matsuda K, Kobayashi M, Oshima Y, Yoshida A, Kawata M, Kubo T. GFP chimeric models exhibited a biphasic pattern of mesenchymal cell invasion in tendon healing. J Cell Physiol. 2007;210:684–91. CrossRef
- Tohyama H, Yasuda K, Uchida H. Is the increase in type III collagen of the patellar tendon graft after ligament reconstruction really caused by “ligamentization” of the graft? Knee Surg Sports Traumatol Arthrosc. 2006;14:1270–7. CrossRef
- The fate and role of bone graft-derived cells after autologous tendon and bone transplantation into the bone tunnel
Journal of Orthopaedic Science
Volume 18, Issue 6 , pp 994-1004
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- 1. Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto, 602-8566, Japan
- 2. Department of Anatomy and Neurobiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan