Experimental study of calcium phosphate cement impregnated with dideoxy-kanamycin B
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
The present study was undertaken to examine whether antibiotic-impregnated calcium phosphate cement (CPC) would provide a valid means of treating osteomyelitis.
The antibiotic used for the impregnation was dideoxy-kanamycin B (DKB), which is available in two forms (powder and liquid). Columnar test specimens (diameter 7 mm, height 14 mm) were prepared by adding the liquid or powdered DKB.
Group A: Three types (6.25-titer, 12.5-titer, 25-titer) of test specimen were prepared by mixing the setting solution and DKB solutions into cement.
Group B: Three types (25-titer, 50-titer, 100-titer) of test specimen were prepared by mixing the setting solution and DKB powder into cement.
Group C: A control specimen was prepared by mixing the setting solution into the cement. The study included a consistency test, setting-time test, compressive strength test, porosity test, and elution test.
The value for the consistency test was >23 mm in all test groups. The results of the setting-time test showed that the setting time became significantly longer as the DKB content increased for groups A group B. Compressive strength decreased as the antibiotic content increased, although all specimens remained sufficiently strong for clinical application. In group A the porosity did not differ significantly depending on the antibiotic content, whereas in group B the porosity increased significantly as the antibiotic content increased. In the elution test using specimens with the same titer (25 titer), the elution efficiency was higher in group A than in group B, and the duration of elution was longer in group A.
Although polymethylmethacrylate (PMMA) has been conventionally used as a drug-delivery system (DDS), the results of the present study indicate that CPC shows better elution efficiency than PMMA. It is thus a promising DDS for the treatment of osteomyelitis.
- Hidaka, N, Yamano, Y, Kadoya, Y, Nishimura, N (2002) Calcium phosphate bone cement for treatment of distal radius fractures: a preliminary report. J Orthop Sci 7: pp. 182-7 CrossRef
- Kita, K, Masada, K, Yasuda, M, Takeuchi, E (1998) Enchondroma protuberance of the phalanx: a case report. J Hand Surg [Am] 28: pp. 1052-4 CrossRef
- Matsuyama, Y, Goto, M, Yoshihara, H, Tsuji, T, Sakai, Y, Nakamura, H (2004) Vertebral reconstruction with biodegradable calcium phosphate cement in the treatment of osteoporotic vertebral compression fracture using instrumentation. J Spinal Disord Tech 17: pp. 291-6 CrossRef
- Sasaki, T, Ishibashi, Y, Katano, H, Nagumo, A, Toh, S (2005) In vitro elution of vancomycin from calcium phosphate cement. J Arthroplasty 20: pp. 1055-9 CrossRef
- Suzuki, M, Tsukeoka, T, Tsuneizumi, Y, Kim, T, Yamanaka, H, Nakamura, H (2004) Mechanical strength and in vitro antibiotic release profile of antibiotics-loaded calcium phosphate bone paste. Rinshou Seikeigeka (Clin Orthop) 39: pp. 309-14
- Kurashima, K, Kurita, H, Hirano, M, de Blieck, JMA, Klein, CPAT, De Groot, K (1995) Calcium phosphate cement: in vitro and in vivo studies of the α-tricalcium phosphate-dicalcium phosphate dibasic-tetracalcium phosphate monoxide system. J Mater Sci Mater Med 6: pp. 340-7 CrossRef
- Asaoka, N, Misago, M, Hirano, M, Takeuchi, H (1999) Mechanical and chemical properties of the injectable calcium phosphate cement. Bioceramics 12: pp. 525-8
- Kokubo, T, Hayashi, T, Sakka, S, Kitsugi, T, Yamamuro, T (1987) Bonding between bioactive glasses, glass-ceramics or ceramics in a simulated body fluid. Yogyo Kyokai Shi 95: pp. 785-91
- Buchholz, HW, Engelbrecht, H (1970) Über die depotwirkung einiger Antibiotika bei Vermischung mit dem Kunstharz Palacos. Chirurg 40: pp. 511-5
- Levin, PD (1975) The effectiveness of various antibiotics in methylmethacrylate. J Bone Joint Surg Br 57: pp. 234-7
- Chapman, MW, Hadley, K (1976) The effect of polymethylmethacrylate and antibiotic combinations on bacterial viability: an in vitro and preliminary in vivo study. J Bone Joint Surg Am 58: pp. 76-81
- Marks, KE, Nelson, CL, Lautenschlager, EP (1976) Antibiotic-impregnated acrylic bone cement. J Bone Joint Surg Am 58: pp. 358-64
- Picknell, B, Mizen, L, Sutherland, R (1977) Antibacterial activity of antibiotics in acrylic bone cement. J Bone Joint Surg Br 59: pp. 302-7
- Ger, E, Dall, D, Miles, T, Forder, A (1977) Bone cement and antibiotics. S Afr Med J 51: pp. 276-9
- Nampei, K (1979) Experimental and clinical studies on the antibacterial activities of acrylic bone cement with antibiotics. Osakashi Igakukai Zasshi (J Osaka City Med Cent) 28: pp. 573-95
- Kinoshita, I (1973) Preventive measures against postoperative infection following prosthetic arthroplasty: study of bone cement mixed with antibiotics. Chuubu Nihon Seikeigeka Saigaigeka Gakkai Zasshi (Cent Jpn J Orthop Traumatol) 16: pp. 899-915
- Kanellakopoulou, K, Gimarellos-Bourboulis, EJ (2000) Carrier systems for the local delivery of antibiotics in bone infections. Drugs 59: pp. 1223-32 CrossRef
- Shinto, Y, Uchida, A, Korkusuz, F, Araki, N, Ono, K (1992) Calcium hydroxyapatite ceramic used as a delivery system for antibiotics. J Bone Joint Surg Br 74: pp. 600-4
- Itokazu, M, Yang, W, Aoki, T, Ohara, A, Kato, N (1998) Synthesis of antibiotic-loaded interporous hydroxyapatite blocks by vacuum method and in vitro drug release testing. Biomaterials 19: pp. 817-9 CrossRef
- Hamanishi, C, Kitamoto, K, Tanaka, S, Otsuka, M, Doi, Y, Kitahashi, T (1996) A self-setting TTCP-DCPD apatite cement for release of vancomycin. J Biomed Mater Res 33: pp. 139-43 CrossRef
- Hasegawa, M, Sudo, A, Komlev, VS, Barinov, SM, Uchida, A (2004) High release of antibiotic from a novel hydroxyapatite with bimodal pore size distribution. J Biomed Mater Res B Appl Biomater 70: pp. 332-9 CrossRef
- Tanaka, A, Ishii, Y (2002) Mechanical properties of the femur after injection of calcium phosphate cement containing arbekacin sulfate and polylactic acid in a rat model of experimental osteoporosis. J Orthop Sci 7: pp. 370-8 CrossRef
- Umezawa, H, Umezawa, S, Tsuchiya, T, Okazaki, Y (1971) 3′,4′-Dideoxy-kanamycin B active against kanamycin-resistant Escherichia coli and Pseudomonas aeruginosa. J Antibiot (Tokyo) 24: pp. 485-7
- Røhl, L, Larsen, E, Linde, F, Odgaard, A, Jørgensen, J (1991) Tensile and compressive properties of cancellous bone. J Biomech 24: pp. 1143-9 CrossRef
- Carter, DR, Spengler, DM (1978) Mechanical properties and composition of cortical bone. Clin orthop 135: pp. 192-217
- Nakazawa, S, Otsuki, M, Nakao, M, Tsukazawa, S, Naha, H, Futaki, K (1974) Bacteriological studies on 3′,4′-dideoxykanamycin B. Chemotherapy 22: pp. 779-84
- Experimental study of calcium phosphate cement impregnated with dideoxy-kanamycin B
Journal of Orthopaedic Science
Volume 12, Issue 3 , pp 281-288
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Industry Sectors
- Author Affiliations
- A1. Department of Orthopaedic Surgery, Yoshimoto Orthopaedic Surgery and Surgical Hospital, 136 Noguchi, Yamatotakada, Nara, 635-0075, Japan
- A2. Department of Orthopaedic Surgery, Nara Medical University, Nara, Japan
- A3. Department of Precision Engineering, Graduate school of Engineering, The University of Tokyo, Tokyo, Japan