JBIC Journal of Biological Inorganic Chemistry

, Volume 16, Issue 2, pp 285–297

The essential role of the Cu(II) state of Sco in the maturation of the CuA center of cytochrome oxidase: evidence from H135Met and H135SeM variants of the Bacillus subtilis Sco

  • Gnana S. Siluvai
  • Michiko Nakano
  • Mary Mayfield
  • Ninian J. Blackburn
Original Paper

DOI: 10.1007/s00775-010-0725-z

Cite this article as:
Siluvai, G.S., Nakano, M., Mayfield, M. et al. J Biol Inorg Chem (2011) 16: 285. doi:10.1007/s00775-010-0725-z

Abstract

Sco is a red copper protein that plays an essential yet poorly understood role in the metalation of the CuA center of cytochrome oxidase, and is stable in both the Cu(I) and Cu(II) forms. To determine which oxidation state is important for function, we constructed His135 to Met or selenomethionine (SeM) variants that were designed to stabilize the Cu(I) over the Cu(II) state. H135M was unable to complement a scoΔ strain of Bacillus subtilis, indicating that the His to Met substitution abrogated cytochrome oxidase maturation. The Cu(I) binding affinities of H135M and H135SeM were comparable to that of the WT and 100-fold tighter than that of the H135A variant. The coordination chemistry of the H135M and H135SeM variants was studied by UV/vis, EPR, and XAS spectroscopy in both the Cu(I) and the Cu(II) forms. Both oxidation states bound copper via the S atoms of C45, C49 and M135. In particular, EXAFS data collected at both the Cu and the Se edges of the H135SeM derivative provided unambiguous evidence for selenomethionine coordination. Whereas the coordination chemistry and copper binding affinity of the Cu(I) state closely resembled that of the WT protein, the Cu(II) state was unstable, undergoing autoreduction to Cu(I). H135M also reacted faster with H2O2 than WT Sco. These data, when coupled with the complete elimination of function in the H135M variant, imply that the Cu(I) state cannot be the sole determinant of function; the Cu(II) state must be involved in function at some stage of the reaction cycle.

Keywords

Copper Sco Cytochrome oxidase Selenomethionine X-ray absorption 

Abbreviations

BCA

Bicinchoninic acid

BSco

Bacillus subtilis Sco

Cox

Cytochrome oxidase

DW

Debye–Waller factor

EXAFS

Extended X-ray absorption fine structure

FT

Fourier transform

I-H135M

Triple mutant M52I/M56I/H135M

I-H135SeM

Triple mutant M52I/M56I/H135SeM

SeM

Selenomethionine

TMPD

Tetramethylphenylenediamine

XAS

X-ray absorption spectroscopy

Supplementary material

775_2010_725_MOESM1_ESM.pdf (255 kb)
Supplementary Information (PDF 255 kb)

Copyright information

© SBIC 2010

Authors and Affiliations

  • Gnana S. Siluvai
    • 1
    • 2
  • Michiko Nakano
    • 1
  • Mary Mayfield
    • 1
  • Ninian J. Blackburn
    • 1
  1. 1.Division of Environmental and Biomolecular SystemsOregon Health and Sciences UniversityBeavertonUSA
  2. 2.Department of Chemistry and BiochemistryMontana State UniversityBozemanUSA

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