JBIC Journal of Biological Inorganic Chemistry

, Volume 8, Issue 7, pp 726–732

The cellular distribution and oxidation state of platinum(II) and platinum(IV) antitumour complexes in cancer cells

Authors

  • Matthew D. Hall
    • Centre for Heavy Metals Research, School of Chemistry F11The University of Sydney
  • Carolyn T. Dillon
    • Centre for Heavy Metals Research, School of Chemistry F11The University of Sydney
  • Mei Zhang
    • Department of Radiation OncologyRoyal Prince Alfred Hospital
  • Philip Beale
    • Department of Medical Oncology, Sydney Cancer CentreRoyal Prince Alfred Hospital
  • Zhonghou Cai
    • Experimental Facilities DivisionArgonne National Laboratory
  • Barry Lai
    • Experimental Facilities DivisionArgonne National Laboratory
  • Anton P. J. Stampfl
    • Experimental Facilities DivisionArgonne National Laboratory
    • Physics DivisionAustralian Nuclear Science and Technology Organisation
    • Centre for Heavy Metals Research, School of Chemistry F11The University of Sydney
Original Article

DOI: 10.1007/s00775-003-0471-6

Cite this article as:
Hall, M.D., Dillon, C.T., Zhang, M. et al. J Biol Inorg Chem (2003) 8: 726. doi:10.1007/s00775-003-0471-6

Abstract

The cellular distribution of platinum in A2780 ovarian cancer cells treated with cisplatin and platinum(IV) complexes with a range of reduction potentials has been examined using elemental analysis (synchrotron radiation-induced X-ray emission). The cellular distribution of platinum(IV) drugs after 24 h is similar to that of cisplatin, consistent with the majority of administered platinum(IV) drugs being reduced. Micro-X-ray absorption near-edge spectra of cells treated with cisplatin and platinum(IV) complexes confirmed the reduction of platinum(IV) to platinum(II). In cells treated, the most difficult to reduce complex, cis,trans,cis-[PtCl2(OH)2(NH3)2], platinum(IV) was detected in the cells along with platinum(II). The observations are in accordance with the relative ease of reduction of the platinum(IV) complexes used and support the requirement of reduction for activation of platinum(IV) complexes.

Keywords

Cellular distributionCisplatinOvarian cancer cellsPlatinum complexesSynchrotron radiation-induced X-ray emission

Abbreviations

en

ethane-1,2-diamine

GM

growth medium

PBS

phosphate buffered saline

RPMI

Roswell Park Memorial Institute

SRIXE

synchrotron radiation-induced X-ray emission

XAFS

X-ray absorption fine structure

XANES

X-ray absorption near-edge spectroscopy

Copyright information

© SBIC 2003